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VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of tumor cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
973-TM) was serially diluted and captured by Mouse Anti-Human TIMP‑3 Monoclonal Antibody (Catalog # MAB973) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human TIMP‑3 Monoclonal Antibody (Catalog # MAB9731) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
973-TM) was serially diluted and captured by Mouse Anti-Human TIMP‑3 Monoclonal Antibody (Catalog # MAB973) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human TIMP‑3 Monoclonal Antibody (Catalog # MAB9731) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
TIMPs form tight inhibitory complexes with MMPs, which are involved in matrix degradation and in tumor invasiveness and metastasis. TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.