Mouse TIMP-2 ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1816
    • Availability
      Product Discontinued

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Mouse TIMP-2 ELISA Kit (Colorimetric) Summary

Description
Quality control test: Standard Curve. Timp2 (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Timp2.
Sensitivity
80 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TIMP2

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Useful for quantification.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.

Kit Components

Components
  1. Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent.
  2. Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For standard/sample (cell culture medium) diluent.
  3. Detection Antibody TIMP-2 (Item F): 2 vial of biotinylated anti-mouse TIMP-2 (each vial is enough to assay half microplate).
  4. HRP-Streptavidin Concentrate (Item G): 8 µl 6,000x concentrated HRP-conjugated streptavidin.
  5. Standards (Item C): 2 vials of recombinant mouse TIMP-2.
  6. Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
  7. TIMP-2 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-mouse TIMP-2.
  8. TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
  9. Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse TIMP-2 ELISA Kit (Colorimetric)

  • CSC-21Ktissue inhibitor of metalloproteinase 2
  • metalloproteinase inhibitor 2
  • TIMP metallopeptidase inhibitor 2
  • TIMP2
  • TIMP-2
  • Tissue inhibitor of metalloproteinases 2

Background

The TIMP2 gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role a

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for TIMP-2 ELISA Kit (KA1816). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Additional TIMP-2 Products

Research Areas for TIMP-2 ELISA Kit (KA1816)

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Blogs on TIMP-2.

Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation
By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa...  Read full blog post.

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Bioinformatics

Gene Symbol TIMP2