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TIMP-2 Antibody (89025) [Unconjugated]


Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 2 more
TIMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB971) overnight at 4 °C. Tissue was stained with more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC

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TIMP-2 Antibody (89025) [Unconjugated] Summary

Chinese hamster ovary cell line CHO-derived recombinant human TIMP‑2
Accession # P16035
Detects human TIMP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human TIMP-1 or recombinant mouse TIMP-1 is observed.
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Read Publications using
MAB971 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIMP-2 Antibody (89025) [Unconjugated]

  • CSC-21Ktissue inhibitor of metalloproteinase 2
  • metalloproteinase inhibitor 2
  • TIMP metallopeptidase inhibitor 2
  • TIMP2
  • TIMP-2
  • Tissue inhibitor of metalloproteinases 2


Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIMP-2 Antibody (MAB971)(13)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Cell-based ELISA, ICC, Immunoprecipitation, Western Blot.

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Cell-based ELISA
Western Blot
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using MAB971 Applications Species
Backe, SJ;Votra, SD;Stokes, MP;Sebesty�n, E;Castelli, M;Torielli, L;Colombo, G;Woodford, MR;Mollapour, M;Bourboulia, D; PhosY-secretome profiling combined with kinase-substrate interaction screening defines active c-Src-driven extracellular signaling Cell reports 2023-05-25 [PMID: 37243593] (Immunoprecipitation, Human) Immunoprecipitation Human
Gerwinn T, Salemi S, Schori LJ et al. Improved contractile potential in detrusor microtissues from pediatric patients with end stage lower urinary tract dysfunction Frontiers in Cell and Developmental Biology 2022-10-04 [PMID: 36268506]
Yang Wang, Qi Yang, Yanli Cheng, Meng Gao, Lei Kuang, Chun Wang Myosin Heavy Chain 10 (MYH10) Gene Silencing Reduces Cell Migration and Invasion in the Glioma Cell Lines U251, T98G, and SHG44 by Inhibiting the Wnt/ beta -Catenin Pathway Medical Science Monitor 2018-12-15 [PMID: 30552850]
Y Yang, Q Li, L Guo MicroRNA?122 acts as tumor suppressor by targeting TRIM29 and blocking the activity of PI3K/AKT signaling in nasopharyngeal carcinoma in�vitro Mol Med Rep, 2018-04-19;0(0):. 2018-04-19 [PMID: 29693120] (Western Blot, Human) Western Blot Human
Angela Armento, Elena I. Ilina, Tony Kaoma, Arnaud Muller, Laurent Vallar, Simone P. Niclou et al. Carboxypeptidase E transmits its anti-migratory function in glioma cells via transcriptional regulation of cell architecture and motility regulating factors International Journal of Oncology 2017-08-01 [PMID: 28656234]
Granule exocytosis is required for platelet spreading: differential sorting of alpha-granules expressing VAMP-7. Blood, 2012-05-15;120(1):199-206. 2012-05-15 [PMID: 22589474] (ICC, Human) ICC Human
Agnieszka Hałoń, Ewa Nowak-Markwitz, Piotr Donizy, Rafał Matkowski, Adam Maciejczyk, Tserenchunt Gansukh et al. Enhanced Immunoreactivity of TIMP-2 in the Stromal Compartment of Tumor as a Marker of Favorable Prognosis in Ovarian Cancer Patients Journal of Histochemistry & Cytochemistry 2012-07-01 [PMID: 22511598]
Yang SW, Cody JJ, Rivera AA, Waehler R, Wang M, Kimball KJ, Alvarez RA, Siegal GP, Douglas JT, Ponnazhagan S Conditionally replicating adenovirus expressing TIMP2 for ovarian cancer therapy. Clin. Cancer Res., 2010-11-29;17(3):538-49. 2010-11-29 [PMID: 21115652] (Western Blot, Human) Western Blot Human
Li MQ, Hou XF, Shao J, Tang CL, Li DJ The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy. Biol. Reprod., 2010-01-14;82(5):968-79. 2010-01-14 [PMID: 20075392] (Cell-based ELISA, Human) Cell-based ELISA Human
Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins. Exp. Hematol., 2009-05-03;37(7):849-56. 2009-05-03 [PMID: 19410025] (ICC, Human) ICC Human
Show All 13 Publications.

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Gene Symbol TIMP2