TIMP-1 Antibody [Unconjugated]

Images

 
Recombinant Human MMP‑2 (0.2 µg/mL, Catalog # 902-MP) activity is measured in the presence of Recombinant Human TIMP-1 (0.1 µg/mL, Catalog # 970-TM) that has been preincubated with increasing concentrations of Goat ...read more
Western blot shows lysates of PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, and SK‑OV‑3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 5 µg/mL ...read more
Simple Western lane view shows lysates of PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, and human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ...read more
Genetic Strategies: Western blot shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line and TIMP-1 knockout SK-OV-3 cell line (KO). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human ...read more
TIMP‑1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) at 1 µg/mL for 1 ...read more
Western blot shows lysates of human lung tissue and human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, B/N, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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TIMP-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-1
Cys24-Ala207
Accession # Q6FGX5
Specificity
Detects human TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse TIMP‑1 and recombinant rat TIMP-1 is observed and less than 1% cross-reactivity with recombinant human (rh) TIMP-2, rhTIMP-3, and rhTIMP‑4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TIMP1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/mL
  • Simple Western 50 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Knockout Validated
  • Neutralization 1 ug/mL
Control
TIMP-1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
AF970 in the following applications:

Publications
Read Publications using
AF970 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIMP-1 Antibody [Unconjugated]

  • CLGI
  • Collagenase inhibitor
  • collagenase inhibitor)
  • EPATIMP-1
  • EPO
  • erythroid potentiating activity
  • Erythroid-potentiating activity
  • Fibroblast collagenase inhibitor
  • FLJ90373
  • HCI
  • metalloproteinase inhibitor 1
  • TIMP metallopeptidase inhibitor 1
  • TIMP1
  • TIMP-1
  • TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity
  • Tissue inhibitor of metalloproteinases 1

Background

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
Applications: WB, Simple Western, IHC, B/N, KO

Publications for TIMP-1 Antibody (AF970)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: IHC-P, In Vivo, Neutralization, Proximity Ligation Assay (PLA), Western Blot.


Filter By Application
IHC-P
(1)
In Vivo
(1)
Neutralization
(2)
Proximity Ligation Assay (PLA)
(1)
Western Blot
(3)
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Human
(8)
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Showing Publications 1 - 8 of 8.
Publications using AF970 Applications Species
CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells Cell Rep, 2019;28(13):3329-3337.e5. 2019 [PMID: 31553904] (Western Blot, Human) Western Blot Human
Ding X, Yang D, Xia L, Chen B, Yu S, Niu Y, Wang M, Li G, Chang C Targeting TR4 nuclear receptor suppresses prostate cancer invasion via reduction of infiltrating macrophages with alteration of the TIMP-1/MMP2/MMP9 signals. Mol Cancer, 2015;14(0):16. 2015 [PMID: 25623427] (IHC-P, Human) IHC-P Human
Thorsen S, Christensen S, Wurtz S, Lundberg M, Nielsen B, Vinther L, Knowles M, Gee N, Fredriksson S, Moller S, Brunner N, Schrohl A, Stenvang J Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective study. BMC Cancer, 2013;13(0):598. 2013 [PMID: 24330623] (Proximity Ligation Assay (PLA), Human) Proximity Ligation Assay (PLA) Human
Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth. Nat. Med., 2011;17(11):1498-503. 2011 [PMID: 22037646] (In Vivo, Human) In Vivo Human
Tyan SW, Kuo WH, Huang CK Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesis. PLoS ONE, 2011;6(1):e15313. 2011 [PMID: 21249190] (Neutralization, Human) Neutralization Human
Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation. Eur. J. Haematol., 2010;84(3):239-51. 2010 [PMID: 19922462] (Neutralization, Human) Neutralization Human
Bittner JG, Wilson M, Shah MB, Albo D, Feig BW, Wang TN Fibroblast-conditioned media promote human sarcoma cell invasion. Surgery, 2009;145(1):42-7. 2009 [PMID: 19081474] (Western Blot, Human) Western Blot Human
Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3. J. Cell Biol., 2006;175(1):179-91. 2006 [PMID: 17030988] (Western Blot, Human) Western Blot Human

Review for TIMP-1 Antibody (AF970) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF970:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen TIMP-1 AF970
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reviewed by:
Anonymous
IHC-Fr Human 07/18/2019
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen human skin sections
SpeciesHuman
LotBUO0418071

Product General Protocols

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Video Protocols

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FAQs for TIMP-1 Antibody (AF970) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF970

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Bioinformatics Tool for TIMP-1 Antibody (AF970)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
07/18/2019
Application: IHC-Fr
Species: Human

Bioinformatics

Gene Symbol TIMP1
Uniprot