Tight Junction Protein 2 Overexpression Lysate (Native)

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Western Blot: Tight junction protein ZO-2 Overexpression Lysate (Adult Normal) [NBL1-16938] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tight junction ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Tight Junction Protein 2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205295 and based on accession number NM_004817. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens tight junction protein 2 (zona occludens 2) (TJP2), transcript variant 1, mRNA.
Gene
TJP2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
133.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Tight Junction Protein 2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Tight Junction Protein 2 Overexpression Lysate (Native)

  • C9DUPq21.11
  • DFNA51
  • DUP9q21.11
  • MGC26306
  • tight junction protein 2 (zona occludens 2)
  • Tight junction protein 2
  • TJP2
  • X104
  • X104tight junction protein ZO-2
  • ZO2
  • ZO-2
  • ZO2Friedreich ataxia region gene X104 (tight junction protein ZO-2)
  • Zona occludens protein 2
  • Zonula occludens protein 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Blogs on Tight Junction Protein 2.

Zonula Occludens (ZO) the Junction Scaffolding Proteins
The Zonula Occludens (ZO) proteins 1,2 and 3, also known as tight junction proteins, are peripheral proteins localizing at junctional sites(1). ZO proteins are known to be scaffolding proteins recruiting various types of proteins to the cytoplasmic...  Read full blog post.

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Bioinformatics

Gene Symbol TJP2