For immunohistology, immunofluorescence and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
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Alternate Names for Tight Junction Protein 1 Antibody
tight junction protein 1 (zona occludens 1)
Tight junction protein 1
tight junction protein ZO-1
zona occludens 1
Zona occludens protein 1
zonula occludens 1 protein
Zonula occludens protein 1
The polyclonal antibody recognizes human zona occludens 1 (ZO-1), an ~220 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Tight Junction Protein 1 Antibody (NBP1-49669). (Showing 1 - 3 of 3 FAQs).
We tested both NBP1-49669 and R-079-100 by WB, but failed to obtain signal. May we ask your suggestion for positive control? We found NBL1-17341 may be an option for R-079-100, but is there any other lysate which can be suggested and is easier to get?
In our catalog, NBP2-10877 as well as NBP2-05057 are the products that are suggested as lysates for ZO-1 tight junction protein antibody (NBP1-49669). You already know NBL1-17341 may be an option for VRL1 Antibody (R-079-100). I do not see any other products in our collection that will go along ZO-1 tight junction protein antibody (NBP1-49669) and VRL1 antibody (R-079-100).
Would you please help confirm if this ZO-1 tight junction protein antibody (cat. no. NBP1-49669) is suitable for ICC?
Yes, this antibody has been validated to be useful in Immunocytochemistry/Immunofluorescence.
The data sheet for the ZO-1 tight junction antibody (NBP1-49669) states to store at 4C. Would it be okay to aliquot and store at -20C or -80C (or perhaps better)? This is an affinity purified antibody in PBS containing 0.1% BSA and 0.02% Sodium Azide, so I would suspect this might be better.
The recommended storage conditions for this antibody are 4C. Under recommended storage conditions, this product is considered stable for at least one year and carries a guarantee for 6 months. Our experience with this antibody is for long term storage at 4C. I would not recommend freezing this antibody. Some antibodies can be very sensitive to freezing and will loose their reactivity if you do so.
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