Recombinant Human Thymidine Kinase 2 His Protein

SDS-Page: Recombinant Human Thymidine Kinase 2 Protein [NBP1-98864] - 15% SDS-PAGE (3ug)

SDS-PAGE: Recombinant Human Thymidine Kinase 2 Protein [NBP1-98864] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human Thymidine Kinase 2 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 34-265 of Human Thymidine Kinase 2
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMVQRRA WPPDKEQEKE KKSVICVEGN IASGKTTCLE FFSNATDVEV LTEPVSKWRN VRGHNPLGLM YHDASRWGLT LQTYVQLTML DRHTRPQVSS VRLMERSIHS ARYIFVENLY RSGKMPEVDY VVLSEWFDWI LRNMDVSVDL IVYLRTNPET CYQRLKKRCR EEEKVIPLEY LEAIHHLHEE WLIKGSLFPM AAPVLVIEAD HHMERMLELF EQNRDRILTP ENRKHCP

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TK2
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 30.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.5M NaCl, 250mM Imidazole, 0.1mM PMSF
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human Thymidine Kinase 2 His Protein

• EC 2.7.1.21
• MTDPS2
• MTTK
• mt-TK
• thymidine kinase 2, mitochondrial

Background

TK2, also known as thymidine kinase 2 mitochondrial, belongs to the DCK/DGK family. Thymidine kinase is an enzyme, a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, It can be found in most living cells. It is present in two forms in mammalian cells, TK1 and TK2. Certain viruses also have genetic information for expression of viral thymidine kinases. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Defects in TK2 are a cause of mitochondrial DNA depletion syndrome type 2 (MTDPS2). A disorder characterized primarily by childhood onset of muscle weakness associated with depletion of mtDNA in skeletal muscle. Recombinant human TK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TK2