Thrombospondin-1 Antibody (A4.1) Summary
Calcium-replete, native purified human TSP from the supernatant of thrombin-activated platelets
N-terminal half of the central stalk-like region of TSP 1 which is recovered as a 50 kDa fragment after chymotryptic digestion of TSP.
It shows no cross-reaction with fibronectin, fibrinogen or Von Willebrand factor.
Ammonium sulfate precipitation
Test in a species/application not listed above to receive a full credit towards a future purchase.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
10mM PBS (pH 7.4) and 0.2% BSA
0.09% Sodium Azide
Ammonium sulfate precipitation
- Western Blot 1 - 2 ug/ml
- Flow Cytometry 1:10 - 1:1000
- Immunofluorescence 1:10 - 1:500
May be useful in Flow, IF, and WB. WB: Detects an approx. 450 kDa (nonreduced), which corresponds to the predicted molecular weight of Thrombospondin. This antibody blocks anti-angiogenic activity of TSP 1 by inhibiting its binding to TSP Receptor / CD36. This antibody inhibits the adhesion of human melanoma G361 cells, keratinocytes, squamous carcinoma cells, and rat smooth muscle cells to TSP 1. It does not inhibit aggregation of thrombin-induced platelets.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Alternate Names for Thrombospondin-1 Antibody (A4.1)
- thrombospondin 1
Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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