Thrombospondin-1 Antibody (A4.1)

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Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554] - Analysis of HUVEC Cells.
Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554] - Analysis of Human Cells in a Non-reducing Gel.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChSpecies Glossary
Applications WB, Flow, IF
Clone
A4.1
Clonality
Monoclonal
Host
Mouse
Concentration
0.2 mg/ml

Order Details

Thrombospondin-1 Antibody (A4.1) Summary

Immunogen
Calcium-replete, native purified human TSP from the supernatant of thrombin-activated platelets
Epitope
N-terminal half of the central stalk-like region of TSP 1 which is recovered as a 50 kDa fragment after chymotryptic digestion of TSP.
Localization
Cell Membrane
Specificity
It shows no cross-reaction with fibronectin, fibrinogen or Von Willebrand factor.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
THBS1
Purity
Ammonium sulfate precipitation
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Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Conjugate
Unconjugated
Buffer
10mM PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Ammonium sulfate precipitation

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Flow Cytometry 1:10 - 1:1000
  • Immunofluorescence 1:10 - 1:500
Application Notes
May be useful in Flow, IF, and WB. WB: Detects an approx. 450 kDa (nonreduced), which corresponds to the predicted molecular weight of Thrombospondin. This antibody blocks anti-angiogenic activity of TSP 1 by inhibiting its binding to TSP Receptor / CD36. This antibody inhibits the adhesion of human melanoma G361 cells, keratinocytes, squamous carcinoma cells, and rat smooth muscle cells to TSP 1. It does not inhibit aggregation of thrombin-induced platelets.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for Thrombospondin-1 Antibody (A4.1)

  • THBS
  • THBS1
  • THBS-1
  • thrombospondin 1
  • Thrombospondin1
  • Thrombospondin-1
  • thrombospondin-1p180
  • TSP-1
  • TSP1thrombospondin-1
  • TSPTSP-1

Background

Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol THBS1
Entrez
Uniprot

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