Thrombospondin-1 Antibody (A4.1)

Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554] - Analysis of HUVEC Cells.

Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554] - Analysis of Human Cells in a Non-reducing Gel.

• Reactivity: Hu, Mu, Rt, Bv, Ch
• Applications: WB, Flow, ICC/IF
• Clone: A4.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Thrombospondin-1 Antibody (A4.1) Summary

• Immunogen: Calcium-replete, native purified human TSP from the supernatant of thrombin-activated platelets
• Epitope: N-terminal half of the central stalk-like region of TSP 1 which is recovered as a 50 kDa fragment after chymotryptic digestion of TSP.
• Localization: Cell Membrane
• Specificity: It shows no cross-reaction with fibronectin, fibrinogen or Von Willebrand factor.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: THBS1
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunofluorescence 1:10 - 1:500
  • Western Blot 1 - 2 ug/ml
• Application Notes: May be useful in Flow, IF, and WB. WB: Detects an approx. 450 kDa (nonreduced), which corresponds to the predicted molecular weight of Thrombospondin. This antibody blocks anti-angiogenic activity of TSP 1 by inhibiting its binding to TSP Receptor / CD36. This antibody inhibits the adhesion of human melanoma G361 cells, keratinocytes, squamous carcinoma cells, and rat smooth muscle cells to TSP 1. It does not inhibit aggregation of thrombin-induced platelets.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for Thrombospondin-1 Antibody (A4.1)

• THBS
• THBS1
• THBS-1
• thrombospondin 1
• Thrombospondin1
• Thrombospondin-1
• thrombospondin-1p180
• TSP-1
• TSP1thrombospondin-1
• TSPTSP-1

Background

Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THBS1
• Entrez:
  • 7057(Human)
  • 56229(Mouse)
  • 364630(Rat)