Thrombospondin-1 Antibody (A4.1)


Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554] - Analysis of HUVEC Cells.
Western Blot: Thrombospondin-1 Antibody (A4.1) [NB600-554] - Analysis of Human Cells in a Non-reducing Gel.

Product Details

Reactivity Hu, Mu, Rt, Bv, ChSpecies Glossary
Applications WB, Flow, ICC/IF
0.2 mg/ml

Order Details

Thrombospondin-1 Antibody (A4.1) Summary

Calcium-replete, native purified human TSP from the supernatant of thrombin activated platelets.
N-terminal half of the central stalk-like region of TSP 1 which is recovered as a 50 kDa fragment after chymotryptic digestion of TSP.
Cell Membrane
It shows no cross-reaction with fibronectin, fibrinogen or Von Willebrand factor.
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
10mM PBS [pH7.4] with 0.2%BSA
0.09% Sodium Azide
0.2 mg/ml
Protein A or G purified


Application Notes
This antibody can be used in Flow Cytometry, Immunofluorescnece, and Western Blot. By Western blot, this antibody detects a band of ~ 450 kDa(nonreduced), which corresponds to the predicted molecular weight of Thrombospondin. This antibody blocks anti-angiogenic activity of TSP 1 by inhibiting its binding to TSP Receptor / CD36. This antibody inhibits the adhesion of human melanoma G361 cells, keratinocytes, squamous carcinoma cells, and rat smooth muscle cells to TSP 1. It does not inhibit aggregation of thrombin-induced platelets. *Optimal dilutions should be determined by the end user.

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for Thrombospondin-1 Antibody (A4.1)

  • THBS
  • THBS1
  • THBS-1
  • thrombospondin 1
  • Thrombospondin1
  • thrombospondin-1p180
  • TSP-1
  • TSP1thrombospondin-1
  • TSPTSP-1


Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol THBS1

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