Themis Antibody [Alexa Fluor® 700]

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

Themis Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant mouse Themis
  Lys122-Lys237
  Accession # Q8BGW0
• Specificity: Detects mouse Themis in direct ELISAs and mouse and rat Themis in Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Themis is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Themis Antibody [Alexa Fluor® 700]

• bA325O24.3
• bA325O24.4
• C6orf190
• C6orf207
• FLJ40584
• Gasp
• GRB2-associated protein
• MGC163388
• protein THEMIS
• signaling phosphoprotein specific for T cells
• SPOT
• Themis
• thymocyte selection associated
• thymocyte selection pathway associated
• thymocyte-expressed molecule involved in selection
• TSEPA

Background

Themis (THymus-Expressed Molecule Involved in Selection; also C6orf190, Gasp and Thylex) is a 72-74 kDa member of the Themis family of proteins. It has restricted expression, being found in double negative #2-4 thymocytes, double positive thymocytes, and CD4⁺ plus CD8⁺ thymocytes and T cells. The molecule is known to constitute part of the TCR signalosome, along with LAT, Grb2 and PLC gamma 1, and is required for ERK and AP-1 activation. Themis appears to contribute to the process of positive selection. In particular, it is needed to prevent cell death during the selection process. Mouse Themis is 636 amino acids (aa) in length. It contains two globular CABIT (Cys-containing, all-beta in Themis) domains (aa 1-260 and 261-520) plus an NLS (aa 555-563). There are four isoform variants. One shows a 25 aa substitution for aa 238-636, a second possesses an alternative start site at Met90 coupled to a 13 aa substitution for aa 589-636, while third and fourth variants contain 7 aa and 17 aa substitutions for aa 589-636. Over aa 122-237, mouse Themis shares 96% and 87% aa identity with rat and human Themis, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.