THEM2 Antibody

Images

 
Western Blot: THEM2 Antibody [NBP1-77049] - HepG2 cell lysate with THEM2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: THEM2 Antibody [NBP1-77049] - Immunofluorescence of THEM2 in human liver tissue with THEM2 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: THEM2 Antibody [NBP1-77049] - Human liver tissue with THEM2 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

THEM2 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human THEM2. The immunogen is located within the last 50 amino acids of THEM2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACOT13
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
THEM2 Peptide (NBP1-77049PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for THEM2 Antibody

  • acyl-CoA thioesterase 13PNAS-27
  • EC 3.1.2.-
  • HT012
  • hypothalamus protein HT012
  • MGC4961
  • THEM2
  • Thioesterase superfamily member 2acyl-coenzyme A thioesterase 13

Background

THEM2, also known as ACOT13, belongs to the hotdog-fold superfamily and possesses thioesterase activity, with greater activity observed with longer chain acyl-CoAs such as myristoyl- and palmitoyl-CoA. THEM2 is highly expressed in several tissues such as heart, kidney, liver and brain and has been shown to be co-localized with beta-tubulin on microtubules. THEM2 interacts with StarD, a protein that plays a key role in fatty acid metabolism, and the addition of StarD to THEM2 increased its catalytic activity, suggesting that THEM2 plays a significant role in the metabolism of fatty acids. At least two isoforms of THEM2 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Diseases for THEM2 Antibody (NBP1-77049)

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Bioinformatics

Gene Symbol ACOT13
Uniprot