TGN46 Antibody

Product Discontinued

TGN46 Antibody Summary

• Immunogen: Antisera to TGN46 were raised by repeated immunisations of sheep with highly purified recombinant human TGN46.
• Marker: TGN Marker
• Specificity: This is specific for TGN46. TGN46 is a heavily glycosylated protein of around 110-120kDa.
• Clonality: Polyclonal
• Host: Sheep
• Gene: TGOLN2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunomicroscopy
  • Western Blot 1:500-1000
• Application Notes: This antibody is useful for Western Blot, Immunofluorescence, and Immunoelectron microscopy. For Immunofluorescence fixation with 3% paraformaldehyde or methanol is recommended, and incubation for 60 minutes at room temperature in blocking buffer (0.1% gelatin in TBS/triton). For Western Blot it is recommended in 5% milk/TBS (+0.1% Tween-20) and incubation for 60 min at room temperature. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 26434594)
• Control:

  TGN38 Overexpression Lysate

Reactivity Notes

This antibody reacts with the human and primate protein. The antibody reactivity varies between species. Other species have not yet been tested. This is the larger size product, 25ug, a smaller size is available: 10ug, product NB110-62093.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: IgG purified

Alternate Names for TGN46 Antibody

• anti-TGN38 homolog antibody
• anti-TGN46 antibody
• anti-TGN48 antibody
• anti-TGOLN2 antibody
• anti-Trans-Golgi network integral membrane protein 2 antibody
• TGN 46
• TGN 48
• TGN 51
• TGN38 homolog
• TGN38
• TGN-38
• TGN46
• TGN-46
• TGN48
• TGN51
• TGOLN 2
• TGOLN2
• trans golgi network 38
• trans golgi network 46
• Trans Golgi network integral membrane protein 2 [Precursor]
• Trans Golgi network integral membrane protein 2
• Trans Golgi network protein (46 48 51kD isoforms)
• Trans golgi network protein 2
• Trans Golgi network protein TGN51
• Trans-Golgi network integral membrane protein 2
• Trans-Golgi network protein 2
• Trans-Golgi network protein TGN51
• TTGN 2
• TTGN2

Background

Sheep anti-human TGN46 recognises a glycoprotein designated TGN46 that is localised primarily in the Trans-Golgi network. TGN46 has been reported as being the best available marker for human trans-Golgi network

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGN46 Antibody (NB110-40767)(1)

Publication using NB110-40767

• Nakajima k, Nozawa T, Minowa-Nozawa A et al. RAB30 regulates PI4kB (phosphatidylinositol 4-kinase beta)-dependent autophagy against group A Streptococcus. Autophagy. 2018-10-05 [PMID: 30290718] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: TGOLN2
• Entrez:
  • 10618(Human)