TGF-beta RIII Antibody (31349) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB
• Clone: 31349
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

TGF-beta RIII Antibody (31349) [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TGF‑β RIII
  Gly21-Asp781
  Accession # AAA67061
• Specificity: Detects human TGF-beta RIII in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant rat MIS RII, recombinant human (rh) TGF-beta RI, rhTGF-beta RII, recombinant mouse TGF-beta RII, or rhTGF-beta RIIb.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta RIII Antibody (31349) [Alexa Fluor® 700]

• betaglycan proteoglycan
• Betaglycan
• BGCAN
• TBRIII
• TGF-beta receptor type 3
• TGF-beta receptor type III
• TGF-beta RIII
• TGFbetaRIII
• TGFBR3
• TGF-bRIII
• TGFR-3
• Transforming growth factor beta receptor III
• transforming growth factor beta receptor type 3
• transforming growth factor, beta receptor III (betaglycan, 300kDa)
• transforming growth factor, beta receptor III

Background

Most cell types express three sizes of receptors for TGF-beta . These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta . The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta . The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction. The Type III receptor binds TGF-beta 2 with the highest affinity. Other TGF-beta isoforms also bind the Type III receptor, but with lower affinities. Cellular responsiveness to TGF-beta 2 appears to be dependent on the presence of the Type III receptor which can interact with the signaling receptor complex. In addition to the transmembrane Type III receptor, a soluble form of the receptor is secreted by some cell types. The physiological role of this soluble receptor remains to be determined. The recombinant TGF-beta soluble receptor Type III binds the TGF-beta isoforms differentially in solution and exhibits TGF-beta antagonistic activities in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.