TGF-beta RII Overexpression Lysate (Native)


Western Blot: TGF beta Receptor II Overexpression Lysate (Adult Normal) [NBL1-16857] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TGF beta Receptor II.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

TGF-beta RII Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC219855 and based on accession number NM_003242. The protein contains a C-terminal DDK tag.
Homo sapiens transforming growth factor, beta receptor II (70/80kDa) (TGFBR2), transcript variant 2, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for TGF-beta RII

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TGF-beta RII Overexpression Lysate (Native)

  • AAT3
  • EC 2.7.11
  • EC
  • FAA3
  • HNPCC6
  • LDS1B
  • LDS2B
  • MFS2
  • RIIC
  • TAAD2
  • tbetaR-II
  • TGF-beta receptor type II
  • TGF-beta receptor type IIB
  • TGF-beta receptor type-2
  • TGF-beta RII
  • TGF-beta type II receptor
  • TGFbetaRII
  • TGFbeta-RII
  • TGFBR2
  • TGF-bRII
  • TGFR-2
  • transforming growth factor beta receptor type IIC
  • transforming growth factor, beta receptor II (70/80kDa) isoform 1
  • transforming growth factor, beta receptor II (70/80kDa) isoform 2
  • transforming growth factor, beta receptor II (70/80kDa)
  • transforming growth factor, beta receptor II (70-80kD)
  • Transforming growth factor-beta receptor type II


This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region compared to variant 1. The resulting protein (isoform B) is shorter than isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
Species: Hu
Applications: WB, ChIP, ICC
Species: Hu, Po, Bv
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IM
Species: Hu, Mu, Rt, Po, Bv, Ch, Xp, Ze
Applications: WB, ELISA, Flow, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
Species: Hu
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Bv, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Bv, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
Species: Hu
Applications: WB

Publications for TGF-beta RII Lysate (NBL1-16857) (0)

There are no publications for TGF-beta RII Lysate (NBL1-16857).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TGF-beta RII Lysate (NBL1-16857) (0)

There are no reviews for TGF-beta RII Lysate (NBL1-16857). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TGF-beta RII Lysate (NBL1-16857) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TGF-beta RII Products

Bioinformatics Tool for TGF-beta RII Lysate (NBL1-16857)

Discover related pathways, diseases and genes to TGF-beta RII Lysate (NBL1-16857). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TGF-beta RII Lysate (NBL1-16857)

Discover more about diseases related to TGF-beta RII Lysate (NBL1-16857).

Pathways for TGF-beta RII Lysate (NBL1-16857)

View related products by pathway.

PTMs for TGF-beta RII Lysate (NBL1-16857)

Learn more about PTMs related to TGF-beta RII Lysate (NBL1-16857).

Research Areas for TGF-beta RII Lysate (NBL1-16857)

Find related products by research area.

Blogs on TGF-beta RII

There are no specific blogs for TGF-beta RII, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our TGF-beta RII Overexpression Lysate (Native) and receive a gift card or discount.


Gene Symbol TGFBR2