TGF-beta RII Antibody

Product Discontinued

TGF-beta RII Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human TGFbeta RII.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGFBR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of TGF Beta Receptor II (I122) pAb in extracts from HepG2 cells(65K) or LOVO cells(100K). Immunocytochemistry/Immunofluorescence and Immunohistochemistry-Frozen were reported in scientific literature.
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  TGF-beta RII Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TGF-beta RII Antibody

• AAT3
• EC 2.7.11
• EC 2.7.11.30
• FAA3
• HNPCC6
• LDS1B
• LDS2B
• MFS2
• RIIC
• TAAD2
• tbetaR-II
• TGF-beta receptor type II
• TGF-beta receptor type IIB
• TGF-beta receptor type-2
• TGF-beta RII
• TGF-beta type II receptor
• TGFbetaRII
• TGFbeta-RII
• TGFBR2
• TGF-bRII
• TGFR-2
• transforming growth factor beta receptor type IIC
• transforming growth factor, beta receptor II (70/80kDa) isoform 1
• transforming growth factor, beta receptor II (70/80kDa) isoform 2
• transforming growth factor, beta receptor II (70/80kDa)
• transforming growth factor, beta receptor II (70-80kD)
• Transforming growth factor-beta receptor type II

Background

A total of three members of the TGFbeta family, TGFbeta1, TGFbeta2 and TGFbeta3, have been identified in mammals. Each is synthesized as a latent precursor that is subsequently cleaved forming the 112 amino acid growth factor which becomes active upon dimerization. TGFbetas mediate their activity by high affinity binding to the type II receptor (TGFbeta RII) transmembrane protein with a cytoplasmic serine-threonine kinase domain. For signaling growth inhibition and early gene responses the type II receptor requires both its kinase activity and association with a TGFbeta-binding protein, designated the type I receptor. Two independent groups have recently described the cloning and sequence analysis of genes encoding TGFbeta type I receptor proteins designated ALK-5 (TbetaR-1) and TSR-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-beta RII Antibody (NB100-91994)(5)

Publications using NB100-91994

• Yang Y, He X, Cheng R et al. Diabetes-induced upregulation of kallistatin levels exacerbates diabetic nephropathy via RAS activation FASEB J. 2020-04-30 [PMID: 32352602] (WB, Mouse)
• Czarzasta K, Koperski L, Segiet A et al. The role of high fat diet in the regulation of MAP kinases activity in left ventricular fibrosis Acta Histochem. 2019-02-04 [PMID: 30733042] (IHC-P, Rat)
• Yokouchi H, Nishihara H, Harada T et al. Immunohistochemical profiling of receptor tyrosine kinases, MED12, and TGF-BRII of surgically resected small cell lung cancer, and the potential of c-kit as a prognostic marker Oncotarget 2017-06-13 [PMID: 28055980] (IHC-P, Human)
• Sakai K, Jawaid S, Sasaki T et al. Transforming Growth Factor-B-Independent Role of Connective Tissue Growth Factor in the Development of Liver Fibrosis Am. J. Pathol. 2014-08-07 [PMID: 25108224]
• Phatnani HP, Guarnieri P, Friedman BA et al. Intricate interplay between astrocytes and motor neurons in ALS. Proc Natl Acad Sci U S A 2013-02-06 [PMID: 23388633] (IHC-Fr, ICC/IF, Mouse)

Reviews for TGF-beta RII Antibody (NB100-91994) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 12/09/2016

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: mouse spinal cord
• Species: Mouse

reviewed by: Verified Customer Simple Western Mouse 08/19/2015

Summary

• Application: Simple Western
• Sample Tested: Ba/F3 (haematopoietic) cells that over-express the mutated version of the thrombopoietin receptor (MPL)
• Species: Mouse

reviewed by: Verified Customer WB Human 09/18/2014

Summary

• Application: Western Blot
• Sample Tested: Lysate from HEK293 cells
• Species: Human

Bioinformatics

• Gene Symbol: TGFBR2
• Entrez:
  • 7048(Human)
  • 21813(Mouse)
  • 81810(Rat)
• Uniprot:
  • P37173()