TGF-beta RII Antibody (25508) [Fluorescein]

NS0 mouse myeloma cell line transfected with TGF-beta RII was stained with Mouse Anti-Human TGF-beta RII Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB241F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Investigating the status of the canonical TGF-beta pathway and levels of Hsp90 in the genetically paired SW480 primary and SW620 secondary tumour-derived colon cancer cell lines. a The secretion of TGF-beta 1 by SW480 and SW620 cells was determined by means of an ELISA to quantify the levels of extracellular TGF-beta 1 in spent culture medium for a given cell number after 12 h incubation. Data represent the average of three independent experiments performed in triplicate and error bars indicate the standard error in the mean. b The levels of TGF-beta RII receptor on the cell surface was determined by flow cytometry using a fluorescein isothiocyanate-conjugated antibody. Data was analysed using FlowJo software and gating carried out according to the IgG1 isotype control. Histograms show a shift in fluorescence for each sample (black line, unshaded) compared to the isotype control (grey shading) and the percentage of positive events is indicated for each sample. Data are representative of three independent experiments showing consistent results. c Intracellular levels of TGF-beta 1 in SW480 and SW620 cells were determined by (i) western blot analysis and compared to a histone loading control. The images are cropped from the full length western blot provided as Additional file 1. (ii) The normalized levels of pre-pro TGF beta 1 from the western blot in (i) were determined by densitometry using ImageJ and calculated according the histone loading control. AU: arbitrary units. The positive control represents commercially obtained recombinant human TGF beta 1 in its active form. d Activation of the TGF-beta canonical pathway was determined by the phosphorylation and nuclear localization of Smad2/3. SW480 and SW620 cells were stained for pSMAD2/3 and nuclei were stained with Hoescht-33342. Immunofluorescence was detected using the Zeiss LSM 510 Meta laser scanning confocal microscope and the images analysed using Axiovision LE 4.7.1 software (Zeiss). (i) The first column shows the level of pSmad2/3 staining in the cells, pseudocoloured to white, while the second column shows a merged image of the nucleus pseudo-coloured to red and the pSMAD2/3 staining pseudocoloured to green. The third column shows frequency scattergrams obtained using colocalisation analysis on Image J,. Scale bars represent 20 μm. (ii) Graphs generated from profiles of the nucleus and pSMAD2/3 within individual cells analysed using Zen Lite Software showing the proportion of total pSMAD2/3 that is found in the nucleus. In all cases, analyses were performed on triplicate images containing at least 10 cells per image e The secretion of Hsp90 beta by SW480 and SW620 cells was quantified by means of an ELISA on spent culture medium after 12 h incubation. The data are representative of two individual experiments performed in triplicate and showing consistent results. f Whole cell lysates from SW480 and SW620 cells were analysed for Hsp90 alpha and Hsp90 beta expression using western blot analysis. Where relevant, data was analysed using GraphPad Prism 4.03 software with errors bars indicating the standard error in the mean and a students t-test was performed to assess statistical significance. (*p < 0.05, **p < 0.01) Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3190-z), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow
• Clone: 25508
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

TGF-beta RII Antibody (25508) [Fluorescein] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TGF-beta RII
  Ile24-Asp159
  Accession # P37173.2
• Specificity: This antibody has been validated by flow cytometry on TGF-beta RII-transfected cells only. In contrast to Catalog # FAB2411F, minimal staining of PBMC lymphocytes was observed with this antibody.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGFBR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta RII Antibody (25508) [Fluorescein]

• AAT3
• EC 2.7.11
• EC 2.7.11.30
• FAA3
• HNPCC6
• LDS1B
• LDS2B
• MFS2
• RIIC
• TAAD2
• tbetaR-II
• TGF-beta receptor type II
• TGF-beta receptor type IIB
• TGF-beta receptor type-2
• TGF-beta RII
• TGF-beta type II receptor
• TGFbetaRII
• TGFbeta-RII
• TGFBR2
• TGF-bRII
• TGFR-2
• transforming growth factor beta receptor type IIC
• transforming growth factor, beta receptor II (70/80kDa) isoform 1
• transforming growth factor, beta receptor II (70/80kDa) isoform 2
• transforming growth factor, beta receptor II (70/80kDa)
• transforming growth factor, beta receptor II (70-80kD)
• Transforming growth factor-beta receptor type II

Background

TGF-beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF-beta RII interacts with TGF-beta RI to form the heteromeric signaling complex that transduces TGF-beta signals. A splice variant of the type II receptor, TGF-beta RIIb, containing a 25 amino acid residue insertion near the N-terminus of the mature protein has also been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-beta RII Antibody (FAB241F)(7)

Publications using FAB241F

• Si‑Xi Liu, Hai‑Rong Xiao, Guo‑Bing Wang, Xiao‑Wen Chen, Chang‑Gang Li, Hui‑Rong Mai et al. Preliminary investigation on the abnormal mechanism of CD4+FOXP3+CD25high regulatory T cells in pediatric B‑cell acute lymphoblastic leukemia Experimental and Therapeutic Medicine 6/19/2018 [PMID: 30116392]
• D Amodio, N Cotugno, G Macchiarul, S Rocca, Y Dimopoulos, MR Castrucci, R De Vito, FM Tucci, AB McDermott, S Narpala, P Rossi, RA Koup, P Palma, C Petrovas Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination J. Immunol., 2017-12-13;0(0):. 2017-12-13 [PMID: 29237774] (Flow Cytometry, Human)
• R Tzach-Nahm, R Nashef, O Fleissig, A Palmon, L Shapira, A Wilensky, G Nussbaum Oral fibroblasts modulate the macrophage response to bacterial challenge Sci Rep, 2017-09-14;7(1):11516. 2017-09-14 [PMID: 28912533] (Flow Cytometry, Human)
• JA de la Mare, T Jurgens, AL Edkins Extracellular Hsp90 and TGF? regulate adhesion, migration and anchorage independent growth in a paired colon cancer cell line model BMC Cancer, 2017-03-16;17(1):202. 2017-03-16 [PMID: 28302086] (Flow Cytometry, Human)
• Wei Cui, Jie Zhou, Nathalie Dehne, Bernhard Brüne Hypoxia induces calpain activity and degrades SMAD2 to attenuate TGF beta signaling in macrophages Cell & Bioscience 2015-12-01 [PMID: 26146544] (FACS, Mouse)
• Ellen J. Beswick, Iryna V. Pinchuk, Rachel B. Earley, David A. Schmitt, Victor E. Reyes Role of gastric epithelial cell-derived transforming growth factor beta in reduced CD4+ T cell proliferation and development of regulatory T cells during Helicobacter pylori infection Infection and Immunity 2011-07-01 [PMID: 21482686]
• Hermitte F, Brunet de la Grange P, Belloc F, Praloran V, Ivanovic Z Very low O2 concentration (0.1%) favors G0 return of dividing CD34+ cells. Stem Cells, 2005-08-25;24(1):65-73. 2005-08-25 [PMID: 16123391] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TGFBR2
• Uniprot:
  • P37173.2()