TGF-beta RI/ALK-5 Antibody (141231) [Phycoerythrin]

NSA Mouse splenocytes were stained with (A) Rat Anti-Mouse TGF-beta RI/ALK-5 PE-conjugated Monoclonal Antibody (Catalog # FAB5871P) or Rat IgG2a isotype control antibody (Catalog # IC006P) and Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

TGF-beta RI/ALK-5 Antibody (141231) [Phycoerythrin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF‑ beta RI/ALK-5
  Ala21-Glu121
  Accession # BAA05023
• Specificity: Detect mouse TGF-beta RI/ALK-5 in flow cytometry. Although this clone detects human and mouse TGF-beta RI/ALK-5 in Western blots, positive results have not been observed on human TGF-beta RI/ALK-5 in flow cytometry. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with rrMIS RII, rhTGF‑ beta RII, rhTGF‑ beta RIIb, or rhTGF‑ beta RIII.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TGFBR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta RI/ALK-5 Antibody (141231) [Phycoerythrin]

• AAT5
• activin A receptor type II-like kinase, 53kD
• activin A receptor type II-like kinase, 53kDa
• Activin receptor-like kinase 5
• ACVRLK4
• ALK-5
• ALK-5ALK5
• EC 2.7.11
• EC 2.7.11.30
• LDS1A
• LDS2A
• Serine/threonine-protein kinase receptor R4
• SKR4
• tbetaR-I
• TGFB1R1
• TGF-beta receptor type I
• TGF-beta receptor type-1
• TGF-beta RI
• TGF-beta type I receptor
• TGFbetaRI
• TGFBR1
• TGF-bRI
• TGFR-1
• transforming growth factor beta receptor I
• transforming growth factor, beta receptor 1
• transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD)
• Transforming growth factor-beta receptor type I

Background

Most cell types express three sizes of receptors for TGF-beta . These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor, originally known as ALK-5 (Activin receptor-like kinase) is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta . Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

1. Miyazono, K. et al. (1994) Adv. In Immunol. 55:181.
2. Massagùe, J. (1998) Ann. Rev. Biochem. 67:753.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-beta RI/ALK-5 Antibody (FAB5871P)(3)

Publications using FAB5871P

• Barnkob, MB;Michaels, YS;André, V;Macklin, PS;Gileadi, U;Valvo, S;Rei, M;Kulicke, C;Chen, JL;Jain, V;Woodcock, VK;Colin-York, H;Hadjinicolaou, AV;Kong, Y;Mayya, V;Mazet, JM;Mead, GJ;Bull, JA;Rijal, P;Pugh, CW;Townsend, AR;Gérard, A;Olsen, LR;Fritzsche, M;Fulga, TA;Dustin, ML;Jones, EY;Cerundolo, V; Semmaphorin 3?A causes immune suppression by inducing cytoskeletal paralysis in tumour-specific CD8+ T cells Nature communications 2024-04-12 [PMID: 38609390] (Flow Cytometry, Mouse)
• A Shukla, C Chen, J Jellusova, CR Leung, E Kao, N Bhat, WW Lin, JR Apgar, RC Rickert Self-reactive B cells in the GALT are actively curtailed to prevent gut inflammation JCI Insight, 2019-07-23;5(0):. 2019-07-23 [PMID: 31335327] (Flow Cytometry, Mouse)
• Joseph M McCune Lin28b Regulates Fetal Regulatory T Cell Differentiation through Modulation of TGF-? Signaling J. Immunol., 2016-10-28;0(0):. 2016-10-28 [PMID: 27793996] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TGFBR1
• Uniprot:
  • BAA05023()