TGF beta 1/2/3 Antibody (1D11) [Phycoerythrin]

PC-3 human prostate cancer cell line was stained with Mouse Anti-TGF-beta 1, 2, 3 PE-conjugated Mono-clonal Antibody (Catalog # IC1835P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Product Discontinued

TGF beta 1/2/3 Antibody (1D11) [Phycoerythrin] Summary

• Immunogen: Bovine bone-derived TGF-beta 1 and TGF-beta 2
• Specificity: Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGFB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF beta 1/2/3 Antibody (1D11) [Phycoerythrin]

• CED
• DPD1
• LAP
• TGFB
• TGFB1
• TGF-beta 1, 2, 3
• TGFbeta
• transforming growth factor, beta 1

Background

TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

1. Ayala A. et al. (1992) FASEB J. 6:A1604.
2. Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
3. Dasch J.R. et al. (1989) J. Immunol. 142:1536.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF beta 1/2/3 Antibody (IC1835P)(4)

Publications using IC1835P

• M Beki?, M Vasiljevi?, D Stojanovi?, V Kokol, D Mihajlovi?, D Vu?evi?, P Uskokovi?, M ?oli?, S Tomi? Phosphonate-Modified Cellulose Nanocrystals Potentiate the Th1 Polarising Capacity of Monocyte-Derived Dendritic Cells via GABA-B Receptor International Journal of Nanomedicine, 2022-07-23;17(0):3191-3216. 2022-07-23 [PMID: 35909813] (Flow Cytometry, Human)
• Henry Kurniawan, Davide G. Franchina, Luana Guerra, Lynn Bonetti, Leticia Soriano Soriano-Baguet, Melanie Grusdat et al. Glutathione Restricts Serine Metabolism to Preserve Regulatory T Cell Function Cell Metabolism 5/1/2020 [PMID: 32213345]
• Yu L, Yang F, Jiang L, Chen Y, Wang K, Xu F, Wei Y, Cao X, Wang J, Cai Z Exosomes with membrane-associated TGF-beta1 from gene-modified dendritic cells inhibit murine EAE independently of MHC restriction. Eur J Immunol, 2013-06-21;43(9):2461-72. 2013-06-21 [PMID: 23716181] (Flow Cytometry, Mouse)
• Notley CA, Brown MA, Wright GP, Ehrenstein MR Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells. J. Immunol., 2011-03-07;186(8):4967-72. 2011-03-07 [PMID: 21383247] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: TGFB1