TGF beta 1/2/3 Antibody (1D11) [Allophycocyanin]

PC-3 human prostate cancer cell line was stained with Mouse Anti-TGF-beta 1, 2, 3 APC-conjugated Mono-clonal Antibody (Catalog # IC1835A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Product Discontinued

TGF beta 1/2/3 Antibody (1D11) [Allophycocyanin] Summary

• Immunogen: Bovine bone-derived TGF-beta 1 and TGF-beta 2
• Specificity: Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGFB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF beta 1/2/3 Antibody (1D11) [Allophycocyanin]

• CED
• DPD1
• LAP
• TGFB
• TGFB1
• TGF-beta 1, 2, 3
• TGFbeta
• transforming growth factor, beta 1

Background

TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

1. Ayala A. et al. (1992) FASEB J. 6:A1604.
2. Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
3. Dasch J.R. et al. (1989) J. Immunol. 142:1536.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF beta 1/2/3 Antibody (IC1835A)(3)

Publications using IC1835A

• A Matsuyama-, B Shojadoost, N Boodhoo, S Raj, M Alizadeh, F Fazel, C Fletcher, J Zheng, B Gupta, MF Abdul-Care, BL Plattner, S Behboudi, S Sharif Activated Chicken Gamma Delta T Cells Are Involved in Protective Immunity against Marek's Disease Viruses, 2023-01-19;15(2):. 2023-01-19 [PMID: 36851499] (Flow Cytometry, Chicken)
• Dutta A, Huang C, Chen T, Lin C, Chiu C, Lin Y, Chang C, He Y IL-10 inhibits neuraminidase-activated TGF-beta and facilitates Th1 phenotype during early phase of infection. Nat Commun, 2015-03-02;6(0):6374. 2015-03-02 [PMID: 25728041] (Flow Cytometry, Mouse)
• Mundkur L, Mukhopadhyay R, Samson S, Varma M, Kale D, Chen D, Shivaprasad S, Sivanandan H, Soman V, Lu X, Kakkar V Mucosal tolerance to a combination of ApoB and HSP60 peptides controls plaque progression and stabilizes vulnerable plaque in Apob(tm2Sgy)Ldlr(tm1Her)/J mice. PLoS ONE, 2013-03-11;8(3):e58364. 2013-03-11 [PMID: 23505495] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: TGFB1