TFII-I Antibody (3E2) - Azide and BSA Free

Western Blot: TFII-I Antibody (3E2) [H00002969-M01] - GTF2I monoclonal antibody (M01), clone 3E2. Analysis of GTF2I expression in human colon.

Immunocytochemistry/Immunofluorescence: TFII-I Antibody (3E2) [H00002969-M01] - Analysis of monoclonal antibody to GTF2I on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: TFII-I Antibody (3E2) [H00002969-M01] - Analysis of monoclonal antibody to GTF2I on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.

Sandwich ELISA: TFII-I Antibody (3E2) [H00002969-M01] - Detection limit for recombinant GST tagged GTF2I is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TFII-I Antibody (3E2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GTF2I (AAH04472.1, 36 a.a. ~ 274 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRKDFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEKMLRDQSAVVVQGLPEGVAFKHPENYDLATLKWILENKAGISFIIKRPFLEPKKHVGGRVMVTDADRSILSPGGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQGSHHSSEGNEGTEMEVPAEG
• Specificity: GTF2I - general transcription factor II, i
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GTF2I
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TFII-I Antibody (3E2) - Azide and BSA Free

• BAP-135,135kD
• BAP135WBS
• Bruton tyrosine kinase-associated protein 135
• BTKAP1
• DIWS
• FLJ38776
• FLJ56355
• general transcription factor II, i
• general transcription factor IIi
• general transcription factor II-I
• GTFII-I
• IB291
• SPINBTK-associated protein 135
• TFII-ISRF-Phox1-interacting protein
• WBSCR6
• Williams-Beuren syndrome chromosome region 6

Background

This gene encodes a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23. Four transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TFII-I Antibody (H00002969-M01)(1)

Publication using H00002969-M01

• Poitras L, Yu M, Lesage-Pelletier C et al. An SNP in an ultraconserved regulatory element affects Dlx5/Dlx6 regulation in the forebrain. Development. 2010-08-11 [PMID: 20702565]

Bioinformatics

• Gene Symbol: GTF2I
• Entrez:
  • 2969(Human)
• OMIM:
  • 601679(Human)
• Uniprot:
  • AAH04472(Human)