Recombinant Human TFF3 Protein

SDS-Page: Human TFF3 Protein [NBP2-35060]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human TFF3 Protein Summary

• Description: A homodimeric protein consisting of two 59 amino acid chains corresponding to TFF3, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds Source: E. coli

  Uniprot ID: Q07654

  Amino Acid Sequence: EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF

• Details of Functionality: Trefoil Factor 3 protein is fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ug/ml, corresponding to a specific activity of > 100 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TFF3
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of TFF3 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 13.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human TFF3 Protein

• HITF, human intestinal trefoil factor10Intestinal trefoil factor
• ITF
• TFF3
• TFI
• trefoil factor 3 (intestinal)

Background

Trefoil Factor 3 (TFF3), also known as ITF and P1.B, plays an important role in epithelial regeneration and wound healing. It is expressed by epithelial goblet cells in the respiratory tract, biliary and breast ducts, small and large intestine, and cardia of the stomach. It is upregulated in response to a range of gastrointestinal epithelial disruptions and induces the migration of biliary, bronchial, and intestinal epithelial cells. Its upregulation is also associated with and enhances tumor cell invasion and metastasis. TFF3 promotes angiogenesis and enhances VEGF upregulation in tumor cells. It can form disulfide-linked dimers or associate into disulfide-linked complexes with other intestinal mucous proteins including FCGBP and MUC-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TFF3
• Uniprot:
  • Q07654()
  • Q07654()
  • Q07654()