TFEB Antibody - BSA Free

Western Blot: TFEB Antibody [NBP2-41167] - Analysis of TFEB in A549 cell lysate with TFEB antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry/ Immunofluorescence: TFEB Antibody - BSA Free [NBP2-41167] - Immunofluorescence of TFEB in human lung tissue with TFEB antibody at 20 ug/mL.

Immunohistochemistry: TFEB Antibody - BSA Free [NBP2-41167] - Immunohistochemistry of TFEB (NT) in human lung tissue with TFEB (NT) antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TFEB Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TFEB Antibody - BSA Free (NBP2-41167) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TFEB. The immunogen is located within amino acids 70 - 120 of TFEB. Amino Acid Squence: SQHQKVREYLSETYGNKF
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TFEB
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 2.5 ug/ml
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TFEB Antibody - BSA Free

• ALPHATFEB
• BHLHE35
• Class E basic helix-loop-helix protein 35
• T-cell transcription factor EB
• TCFEB
• TCFEBbHLHe35
• TFEB
• transcription factor EB

Background

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TFEB