TFB2M Antibody

Images

 
Western Blot: TFB2M Antibody [NBP2-20608] - Expression in transfected 293T cell line by TFB2M polyclonal antibody. A: Non-transfected lysate. B: TFB2M transfected lysate. 12% SDS PAGE gel, diluted at 1:500.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TFB2M Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TFB2M. The exact sequence is proprietary.
Localization
Mitochondrion
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TFB2M
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TFB2M Antibody

  • dimethyladenosine transferase 2, mitochondrial
  • EC 2.1.1.-
  • FLJ22661
  • FLJ23182
  • HCV NS5A-transactivated protein 5
  • Hepatitis C virus NS5A-transactivated protein 5
  • Hkp1
  • h-mtTFB
  • h-mtTFB2
  • hTFB2M
  • Mitochondrial 12S rRNA dimethylase 2
  • Mitochondrial transcription factor B2
  • mtTFB2
  • NS5ATP5
  • S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2
  • transcription factor B2, mitochondrial

Background

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TFB2M
Uniprot