mtRNA polymerase Antibody

Immunocytochemistry/Immunofluorescence: mtRNA polymerase Antibody [NBP1-90126] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: mtRNA polymerase Antibody [NBP1-90126] - Staining of human skeletal muscle shows strong granular cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: mtRNA polymerase Antibody [NBP1-90126] - Staining of human testis shows strong granular cytoplasmic positivity in Leydig cells.

Immunohistochemistry-Paraffin: mtRNA polymerase Antibody [NBP1-90126] - Staining of human prostate shows strong granular positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: mtRNA polymerase Antibody [NBP1-90126] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

mtRNA polymerase Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ELVYVLFMVKDAGLTPDLLSYAAALQCMGRQDQDAGTIERCLEQMSQEGLKLQALFTAVLLSEEDRATVLKAVHKVKPTFSLPPQLPPPVNTSKLLRDVYAKDGRVSYPKLHLPLKTLQCLFEKQLH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLRMT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  mtRNA polymerase Protein (NBP1-90126PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23070475)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for mtRNA polymerase Antibody

• APOLMTEC 2.7.7.6
• DNA-directed RNA polymerase, mitochondrial
• EC 2.7.7.6
• h-mtRPOL
• mtRNA polymerase
• MTRNAP
• MtRPOL
• POLRMT
• polymerase (RNA) mitochondrial (DNA directed)

Background

POLRMT, also known as DNA-directed RNA polymerase, mitochondrial, is a 246 amino acid protein that is 28 kDa, catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. This protein is currently being studied for research on autosomal dominant progressive external ophthalmoplegia, essential thrombocythemia, ophthalmoplegia, immunodeficiency, and malaria. This protein has also shown to have interactions with TFB1M, NFKB2, ICT1, TFB2M, TFAM, and over 200 other proteins in ATP/ITP metabolism, mitochondrial gene expression, transcription from mitochondrial promoters, transcription, RNA polymerase I, RNA polymerase III, and mitochondrial transcription, gene expression, and mitochondrial transcription initiation pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mtRNA polymerase Antibody (NBP1-90126)(2)

Publications using NBP1-90126

• Sotgia F, Whitaker-Menezes D, Martinez-Outschoorn UE et al. Mitochondria fuel breast cancer metabolism: Fifteen markers of mitochondrial biogenesis label epithelial cancer cells, but are excluded from adjacent stromal cells. Cell Cycle 2012-12-01 [PMID: 23172368] (IF/IHC, Human)
• Salem AF, Whitaker-Menezes D, Howell A et al. Mitochondrial biogenesis in epithelial cancer cells promotes breast cancer tumor growth and confers autophagy resistance. Cell Cycle 2012-11-15 [PMID: 23070475]

Bioinformatics

• Gene Symbol: POLRMT
• Entrez:
  • 5442(Human)
• Uniprot:
  • O00411(Human)