TET2 Antibody (CL6873) - Azide and BSA Free

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Orthogonal Strategies: Analysis in human placenta and pancreas tissues using NBP3-44224 antibody. Corresponding TET2 RNA-seq data are presented for the same tissues.
Staining of human pancreas shows no nuclear positivity in exocrine glandular cells as expected.
Staining of human placenta shows moderate nuclear positivity in a subset of trophoblastic cells.
Staining of U-2 OS cells using the Anti-TET2 monoclonal antibody) .
Staining of human uterine cervix shows moderate nuclear positivity in squamous epithelial cells.
Staining of human tonsil shows weak to moderate nuclear positivity in squamous epithelial cells.
Analysis in human cell line HL-60.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL6873
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH
Validated by:
       

Orthogonal Strategies

 

Order Details

TET2 Antibody (CL6873) - Azide and BSA Free Summary

Immunogen
This antibody was generated using a synthetic peptide of Q6N021, with the exact immunogen sequence remaining proprietary.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TET2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for TET2 Antibody (CL6873) - Azide and BSA Free

  • FLJ20032
  • KIAA1546
  • MGC125715
  • Nbla00191
  • tet methylcytosine dioxygenase 2
  • TET2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TET2