Testican 1/SPOCK1 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Testican 1/SPOCK1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Testican 1/SPOCK1
  Arg22-Trp439
  Accession # Q08629
• Specificity: Detects human Testican 1/SPOCK1 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Testican 2 is observed and less than 2% cross-reactivity with rhTestican 3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SPOCK1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Testican 1/SPOCK1 Antibody [Unconjugated]

• FLJ37170
• Protein SPOCK
• sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1
• SPOCK1
• SPOCKcwcv and kazal-like domains proteoglycan (testican)
• Testican 1
• TIC1
• TICN1

Background

Testican 1, encoded by the SPOCK1 gene, is a proteoglycan first identified in human seminal plasma (1). The cDNAs from the human testis and mouse brain indicate 95% identity between the deduced amino acid sequences from the two species, predicting a conserved function (2, 3). Human Testican 1 is able to inhibit attachment of Neuro-2a cells and their ability to form neurite extensions (4). At R&D Systems, rhTestican 1 has also been shown to be able to enhance neurite outgrowth of E18 rat embryonic hippocampal neurons. Testican 1 contains Ca²⁺-binding domain and the C-terminal acidic domain with putative glycosaminoglycan attachment sites (5). In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. The N-terminal region, which is unique to testicans, is responsible for the inhibition of testican 1 towards MMP-14 (MT1-MMP, a metalloprotease) activation of MMP-2 (6). The thyropin domain may be responsible for the inhibition of testican 1 towards cathepsin L, a cysteine protease (5). The follistatin-like domain with a six cysteine Kazal-like motif may inhibit serine proteases. The purified rhTestican 1 is capable of inhibiting rhMMP-14 and rhCathepsin L (R&D Systems, Catalog # 918-MP and 952-CY) in assays using the fluorogenic peptide substrates (R&D Systems, Catalog # ES001 and ES008).

1. Bonnet, F. et al. (1992) Biochem. J. 288:565.
2. Alliel, P.M. et al. (1993) Eur. J. Biochem. 214:347.
3. Bonnet, F. et al. (1996) J. Biol. Chem. 271:565.
4. Marr, H.S. and C.-J.S Edgell (2003) Matrix Biol. 22:259.
5. Bocock, J.P. et al. (2003) Eur. J. Biochem. 270:4008.
6. Nakada, M. et al. (2001) Cancer Res. 61:8896.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Testican 1/SPOCK1 Antibody (AF2327)(1)

Publication using AF2327

• Toru Takahata, Yusuke Komatsu, Akiya Watakabe, Tsutomu Hashikawa, Shiro Tochitani, Tetsuo Yamamori Differential Expression Patterns of occ1-Related Genes in Adult Monkey Visual Cortex Cerebral Cortex 2009-08-01 [PMID: 19073625] (In Situ Hybridization, Primate - Chlorocebus aethiops (African Green Monkey))

Bioinformatics

• Gene Symbol: SPOCK1
• Uniprot:
  • Q08629()