Testican 2/SPOCK2 Antibody

Western blot shows lysates of rat lung tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Testican 2/SPOCK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2328) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Testican 2/SPOCK2 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Testican 2/SPOCK2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Testican 2/SPOCK2
  Glu23-Trp424
  Accession # Q92563
• Specificity: Detects human, mouse, and rat Testican 2/SPOCK2 in direct ELISAs and human Testican 2/SPOCK2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SPOCK2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Testican 2/SPOCK2 Antibody

• FLJ97039
• KIAA0275
• KIAA0275CWCV, and Kazal-like domains proteoglycan 2
• sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2
• SPOCK2
• Testican 2

Background

Testican 2 (also SPOCK2) is a 60 - 140 kDa, secreted multidomain proteoglycan that belongs to the BM-40/SPARC family of extracellular proteins. It is expressed by neurons (both excitatory and GABAergic) plus brain endothelial cells, and appears to abrogate testican 1 and 3 mediated inhibition of MT1- and MT3-MMP activity. Mature human Testican 2 is 402 amino acids (aa) in length (aa 23 - 424). It contains a unique 66 aa N terminus, followed by a Kazal-like follistatin domain (aa 89-192), a collagen-binding domain (aa 210-219), a Ca++ binding region, one thyroglobulin-like segment (aa 310-376) and an acidic C-terminus that contains two potential GAG attachment sites at Ser383 and Ser388. The wide variation in native MW reflects the capabilities of differing cells to add glycan adducts to the molecule. Typically, it appears to lie belween 80-125 kDa. There are potential splice variants. One shows an alternative start site at Met110, a second shows a deletion of aa 64-66, while a third contains a 14 aa substitution for aa 64-242. Mature human Testican 2 is 94% aa identical to mouse and rat Testican 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPOCK2
• Uniprot:
  • Q92563()