TEM4 Antibody - BSA Free

Western Blot: TEM4 Antibody [NBP1-77314] - Analysis of TEM4 in Jurkat cell lysate with TEM4 antibody at (A) 0.5 and (B) 1 ug/mL.

Immunohistochemistry-Paraffin: TEM4 Antibody [NBP1-77314] - Human spleen tissue with TEM4 antibody at 2.5 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TEM4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TEM4 Antibody - BSA Free (NBP1-77314) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 18 amino acid synthetic peptide near the center of human TEM4. The immunogen is located within amino acids 910 - 960 of TEM4. Amino Acid Squence: KKRPARSSHQELRRDEG
• Predicted Species: Mouse (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARHGEF17
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-1 ug/ml
• Theoretical MW: 200 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  TEM4 Peptide (NBP1-77314PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TEM4 Antibody - BSA Free

• 164 kDa Rho-specific guanine-nucleotide exchange factor
• FLJ90019
• KIAA0337P164RHOGEF
• p164RhoGEF
• p164-RhoGEFRho-specific guanine-nucleotide exchange factor 164 kDa
• Rho guanine nucleotide exchange factor (GEF) 17
• TEM4rho guanine nucleotide exchange factor 17
• Tumor endothelial marker 4

Background

Rho GTPases, which are activated by specific guanine-nucleotide exchange factors (GEFs), play pivotal roles in several cellular functions. TEM4, encoding a protein containing 1510 amino acids, contains a RhoGEF-specific Dbl homology (DH) domain but lacks their typical pleckstrin homology domain. TEM4 is a Rho-specific GEF with novel structural and regulatory properties and predominant expression in the heart. It couples tyrosine kinase signals with the activation of the rho/rac GTPases, thus leading to cell differentiation and/or proliferation. Elevated levels of TEM4, TEM5, TEM6, TEM7 and TEM7R were also raised in breast cancer tissues. TEM4 could also prove to be useful targets therapeutically.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TEM4 Antibody (NBP1-77314)(1)

Publication using NBP1-77314

• Zhao X. Identification and Characterization of Protein Kinase C Substrates in Human Breast Cells. Thesis. 2014-10-01 (WB, Human)
  
  Details:
  TEM4 antibody used in WB for confirming shRNA reagent knockdown of TEM4 in MCF-10A cells (Figure 4.16). The antibody also used on MCF-10A cells transiently transfected with each CEP4 (A/A or D/D) or the vector control (Figure 4.15).

Bioinformatics

• Gene Symbol: ARHGEF17
• Uniprot:
  • Q96PE2(Human)