TEM4 Antibody

Images

 
Western Blot: TEM4 Antibody [NBP1-77314] - Analysis of TEM4 in Jurkat cell lysate with TEM4 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunohistochemistry: TEM4 Antibody [NBP1-77314] - Staining of human brain cortex. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western Blot: TEM4 Antibody [NBP1-77314] - Jurkat Cell lysate with TEM4 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry-Paraffin: TEM4 Antibody [NBP1-77314] - Human spleen tissue with TEM4 antibody at 2.5 ug/ml.
Immunohistochemistry-Paraffin: TEM4 Antibody [NBP1-77314] - Staining of human heart. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunohistochemistry-Paraffin: TEM4 Antibody [NBP1-77314] - Staining of human kidney. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TEM4 Antibody Summary

Immunogen
Antibody was raised against a 18 amino acid synthetic peptide near the center of human TEM4. The immunogen is located within amino acids 910 - 960 of TEM4.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARHGEF17
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence 20 ug/mL
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
TEM4 Peptide (NBP1-77314PEP)
Publications
Read Publication using
NBP1-77314 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TEM4 Antibody

  • 164 kDa Rho-specific guanine-nucleotide exchange factor
  • FLJ90019
  • KIAA0337P164RHOGEF
  • p164RhoGEF
  • p164-RhoGEFRho-specific guanine-nucleotide exchange factor 164 kDa
  • Rho guanine nucleotide exchange factor (GEF) 17
  • TEM4rho guanine nucleotide exchange factor 17
  • Tumor endothelial marker 4

Background

Rho GTPases, which are activated by specific guanine-nucleotide exchange factors (GEFs), play pivotal roles in several cellular functions. TEM4, encoding a protein containing 1510 amino acids, contains a RhoGEF-specific Dbl homology (DH) domain but lacks their typical pleckstrin homology domain. TEM4 is a Rho-specific GEF with novel structural and regulatory properties and predominant expression in the heart. It couples tyrosine kinase signals with the activation of the rho/rac GTPases, thus leading to cell differentiation and/or proliferation. Elevated levels of TEM4, TEM5, TEM6, TEM7 and TEM7R were also raised in breast cancer tissues. TEM4 could also prove to be useful targets therapeutically.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TEM4 Antibody (NBP1-77314)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Filter By Species
Human
(1)
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Showing Publication 1 - 1 of 1.
Publication using NBP1-77314 Applications Species
Zhao X. Identification and Characterization of Protein Kinase C Substrates in Human Breast Cells. Thesis. 2014 Oct 01 (WB, Human)

Details:
TEM4 antibody used in WB for confirming shRNA reagent knockdown of TEM4 in MCF-10A cells (Figure 4.16). The antibody also used on MCF-10A cells transiently transfected with each CEP4 (A/A or D/D) or the vector control (Figure 4.15).
WB Human

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WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional TEM4 Products

TEM4 NBP1-77314

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Bioinformatics

Gene Symbol ARHGEF17
Uniprot