TEF1 Antibody (13112)

Western Blot: TEF1 Antibody (13112) [NBP2-43581] - Analysis of whole cell extracts and extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TEF1 antibody [13112] at a dilution of 1:500.

Immunocytochemistry/Immunofluorescence: TEF1 Antibody (13112) [NBP2-43581] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TEF1 protein stained by TEF1 antibody [GT13112] diluted at 1:400. Red: Phalloidin, a F-actin marker, diluted at 1:200.

Immunoprecipitation: TEF1 Antibody (13112) [NBP2-43581] - Immunoprecipitation of TEF1 protein from HeLa nuclear extracts using 5 ug of TEF1 antibody [13112]. Western blot analysis was performed using TEF1 antibody [13112] EasyBlot anti-Mouse IgG was used as a secondary reagent.

• Reactivity: Hu, Po, Ch
• Applications: WB, ICC/IF, IP
• Clone: 13112
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TEF1 Antibody (13112) Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human TEF1. The exact sequence is proprietary.
• Predicted Species: Porcine (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TEAD1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for TEF1 Antibody (13112)

• NTEF-1
• Protein GT-IIC
• TCF13
• TCF-13
• TEA domain family member 1 (SV40 transcriptional enhancer factor)
• TEAD1
• TEAD-1
• TEF1

Background

TEF1 binds specifically and cooperatively to the SPH and GT IIC quot enhansons quot; (GTGGAATGT) and activates transcription in vivo in a cell specific manner. The activation function appears to be mediated by a limiting cell specific transcriptional intermediary factor (TIF). It is involved in cardiac development and also binds to the MCAT motif.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TEAD1