TEAD4 Antibody - BSA Free

Immunohistochemistry-Paraffin: TEAD4 Antibody [NBP1-32765] - SW480 xenograft, using TEAD4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: TEAD4 Antibody [NBP1-32765] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TEAD4 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/ Immunofluorescence: TEAD4 Antibody [NBP1-32765] - TEAD4 antibody detects TEAD4 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TEAD4 stained by TEAD4 antibody (NBP1-32765) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 um.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TEAD4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TEAD4 Antibody - BSA Free (NBP1-32765) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TEAD4. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TEAD4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for TEAD4 Antibody - BSA Free

• EFTR-2
• hRTEF-1B
• related transcription enhancer factor 1B
• RTEF1RTEF-1
• TCF13L1MGC9014
• TEA domain family member 4TEF3
• TEAD-4
• TEF-3
• TEFR-1
• Transcription factor 13-like 1
• Transcription factor RTEF-1
• transcriptional enhancer factor 1-related
• transcriptional enhancer factor 3
• transcriptional enhancer factor TEF-3

Background

This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for TEAD4 Antibody (NBP1-32765) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Feng Li WB Human 11/18/2019

Summary

• Application: Western Blot
• Sample Tested: 293 cell extract
• Species: Human
• Lot: 42789

Bioinformatics

• Gene Symbol: TEAD4