TDP1 Antibody (OTI4H1)

Western Blot: TDP1 Antibody (4H1) [NBP2-01376] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TDP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TDP1.

• Reactivity: Hu
• Applications: WB
• Clone: OTI4H1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.78 mg/ml

TDP1 Antibody (OTI4H1) Summary

• Description: Novus Biologicals Mouse TDP1 Antibody (OTI4H1) (NBP2-01376) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human TDP1(NP_060789) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TDP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 68.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.78 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TDP1 Antibody (OTI4H1)

• EC 3.1.4
• EC 3.1.4.-
• FLJ11090
• MGC104252
• SCAN1
• Tyr-DNA phosphodiesterase 1
• tyrosyl-DNA phosphodiesterase 1

Background

The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TDP1
• Entrez:
  • 55775(Human)