TCR beta Antibody (H57-597) [FITC]

Product Discontinued

TCR beta Antibody (H57-597) [FITC] Summary

• Immunogen: Affinity purified TCR from DO-11.10 T cell hybridoma
• Specificity: The Armenian hamster monoclonal antibody H57-597 reacts specifically with beta subunit of TCR alpha/beta. This antibody does not crossreact with TCR gamma/delta type and has been used as TCR alpha/beta phenotypic marker.
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: Tcrb
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 2 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • In vitro assay

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: IgG purified

Notes

Protect conjugated forms from light.

Alternate Names for TCR beta Antibody (H57-597) [FITC]

• T cell receptor beta chain
• TCRB
• TCRbeta
• Tib

Background

The alpha beta TCR is expressed on T lymphocytes of all mouse strains tested. (2) The H57-597 monoclonal antibody does not react with gammadelta TCR-bearing cells. (2) Plate-bound or soluble H57-597 activates alpha beta TCR bearing T cells. (2) In vitro and in vivo, H57-597 can induce immature thymocytes to undergo apoptosis. (3,4) In vivo administration of H57-597 has been shown to deplete alpha beta TCR-bearing cells to near completion and prevent graft rejection. (5)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Tcrb