TBLR1 Antibody

Western Blot: TBLR1 Antibody [NB100-56501] - analysis of the two forms of TBLR1 in 20 ugs of human brain cell lysate using this antibody.

Product Discontinued

TBLR1 Antibody Summary

• Immunogen: This antibody was developed against a synthetic peptide of human TBLR1, between amino acids 200-250.
• Specificity: A 61 kDa band and a 54 kDa band are observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TBL1XR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3 ug/ml
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Predicted to react with Mouse and Rat based on BLAST results.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for TBLR1 Antibody

• C21
• DC42
• F-box-like/WD repeat-containing protein TBL1XR1
• FLJ12894
• IRA1Transducin beta-like 1X-related protein 1
• Nuclear receptor corepressor/HDAC3 complex subunit TBLR1
• nuclear receptor co-repressor/HDAC3 complex subunit
• TBLR1TBL1-related protein 1
• transducin (beta)-like 1 X-linked receptor 1
• transducin (beta)-like 1X-linked receptor 1

Background

Chromatin organization plays a fundamental role in regulating transcription in eukaryotic cells. The nuclear receptor corepressor (N-CoR) and silencing mediator of retinoid and thyroid homone receptors (SMRT) play a role in diverse transcriptional repression pathways. N-CoR and SMRT each exist in large protein complexes, and are thought to mediate repression, at least in part, by their ability to recruit histone deacetylases to generate repressive chromatin. HDAC3 is the main HDAC that associates with N-CoR and SMRT complexes, and its presence is thought to be essential for repression. TBLR1 is a WD-40 repeat protein that also associates with N-CoR and SMRT complexes. Although TBLR1 is thought to be a core component of both the N-CoR and SMRT complexes, its role in these complexes remains to be elucidated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TBLR1 Antibody (NB100-56501)(1)

Publication using NB100-56501

• Zhou W, He Y, Rehman AU et al. Loss of function of NCOR1 and NCOR2 impairs memory through a novel GABAergic hypothalamus-CA3 projection. Nat. Neurosci. 2019-02-01 [PMID: 30664766] (WB, Human)

Bioinformatics

• Gene Symbol: TBL1XR1
• Entrez:
  • 79718(Human)
• Uniprot:
  • Q9BZK7(Human)