TBC1D32 Antibody

Western Blot: TBC1D32 Antibody [NBP1-90777] - Analysis in human cell line HDLM-2.

Immunocytochemistry/Immunofluorescence: TBC1D32 Antibody [NBP1-90777] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: TBC1D32 Antibody [NBP1-90777] - Staining of human liver shows no positivity in hepatocytes as expected.

Western Blot: TBC1D32 Antibody [NBP1-90777] - Analysis in human cell line HEK 293.

Immunohistochemistry-Paraffin: TBC1D32 Antibody [NBP1-90777] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: TBC1D32 Antibody [NBP1-90777] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: TBC1D32 Antibody [NBP1-90777] - Staining of human testis shows weak cytoplasmic positivity in cells in seminiferous ducts.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TBC1D32 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SVERNHVLVRINLVGGPLERILPPRLLEKSDNPYPWPMFSSYPLPNCYLSDITRNAGIKQDNDLDKLLLCLKISDKQTEWIENCQRQFCKMMKAKPDIISGEALIELLEKFVLHLTESPSECYFPSVEYTATDANVKNE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: C6ORF170
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TBC1D32 Recombinant Protein Antigen (NBP1-90777PEP)

Reactivity Notes

Reactivity reported in mouse normal lung tissue section from a verified customer reveiw.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TBC1D32 Antibody

• bA301B7.2
• bA57L9.1
• BROMI
• C6orf170
• C6orf171
• chromosome 6 open reading frame 170
• dJ310J6.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for TBC1D32 Antibody (NBP1-90777) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human and Mouse.

reviewed by: Verified Customer IHC-P Human and Mouse 02/24/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Adult lung,Salivary gland tissue
• Species: Human and Mouse

Comments

• Comments: Nice staining of IHC-P on human and mouse tissue with this antibody at a dilution 1:50. The section was pre-treated by heat mediated antigen retrieval with sodium citrate buffer (pH6).

Bioinformatics

• Gene Symbol: C6ORF170