Recombinant Human TAK1 GST (N-Term) Protein

SDS-Page: Recombinant Human TAK1 Protein [H00006885-P01]

Functional: Recombinant Human TAK1 Protein [H00006885-P01]

• Reactivity: Hu
• Applications: WB, ELISA, MA, PAGE, AP, Enzyme Activity

Recombinant Human TAK1 GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-579 of Human MAP3K7
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MSTASAASSSSSSSAGEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPCQYSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLESKLLKNQAKQQSESGRLSLGASRGSSVESLPPTSEGKRMSADMSEIEARIAATTGNGQPRRRSIQDLTVTGTEPGQVSSRSSSPSVRMITTSGPTSEKPTRSHPWTPDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSKESMAVFEQHCKMAQEYMKVQTEIALLLQRKQELVAELDQDEKDQQNTSRLVQEHKKLLDENKSLSTYYQQCKKQLEVIRSQQQKRQGTS

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: MAP3K7
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Kinase Assay
  • Protein Array
  • SDS-Page
  • Western Blot
• Theoretical MW: 89.43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human TAK1 GST (N-Term) Protein

• EC 2.7.11
• EC 2.7.11.25
• MAP3K7
• MEKK7
• mitogen-activated protein kinase kinase kinase 7
• TAK1
• TAK1/MAP3K7
• TAK1TGF1a
• TGF1a
• TGF-beta activated kinase 1
• TGF-beta-activated kinase 1
• Transforming growth factor-beta-activated kinase 1

Background

MAP3K7( AAH17715, 1 a.a. - 580 a.a.) recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for TAK1 Recombinant Protein (H00006885-P01)(4)

Publications using H00006885-P01

• Huy Q Le, Matthew A Hill, Ines Kollak, Martina Keck, Victoria Schroeder, Johannes Wirth, Wioletta Skronska‐Wasek, Eva Schruf, Benjamin Strobel, Heiko Stahl, Franziska E Herrmann, Alexandre R Campos, Jun Li, Karsten Quast, Dagmar Knebel, Coralie Viollet, Matthew J Thomas, David Lamb, James P Garnett An EZH2‐dependent transcriptional complex promotes aberrant epithelial remodelling after injury EMBO Reports 2021-07-05 [PMID: 34224201]
• Salazar L, Kashiwada T, Krejci P et al. Fibroblast Growth Factor Receptor 3 Interacts with and Activates TGFb-Activated Kinase 1 Tyrosine Phosphorylation and NFkB Signaling in Multiple Myeloma and Bladder Cancer. PLoS One. 2014-01-23 [PMID: 24466111]
• Liu X, Yao M, Li N et al. CaMKII promotes TLR-triggered proinflammatory cytokine and type I interferon production by directly binding and activating TAK1 and IRF3 in macrophages. Blood. 2008-09-25 [PMID: 18818394]
• Sorrentino A, Thakur N, Grimsby S et al. The type I TGF-beta receptor engages TRAF6 to activate TAK1 in a receptor kinase-independent manner. Nat Cell Biol. 2008-10-01 [PMID: 18758450]

Bioinformatics

• Gene Symbol: MAP3K7