TAF5L Antibody

Western blot shows lysates of RPMI 8226 human multiple myeloma cell line, 293T human embryonic kidney cell line, and U2OS human osteosarcoma cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear (Nuc) extracts. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human TAF5L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6785) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TAF5L at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

TAF5L Antibody Summary

• Immunogen: E. coli-derived recombinant human TAF5L
  Met1-Tyr189
  Accession # O75529
• Specificity: Detects human TAF5L in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TAF1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TAF5L
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TAF5L Antibody

• PAF65B
• PAF65BPAF65-beta
• PCAF associated factor 65 beta
• PCAF-associated factor 65 beta
• TAF5L
• TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDasubunit 5L
• TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associatedfactor, 65 kD
• TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associatedfactor, 65kDa

Background

TAF5L (TBP-Associated Factors 5-Like; also RNA polymerase II p300-associated factor 65 kDa subunit 5L, PACF65 beta , PAF65 beta and STAF65 beta ) is a 64-66 kDa member of the TAF5 family of proteins. It is a nuclear protein that forms part of the PCAF, STAGA and TFTC histone acetylase complexes. The acetylases within these complexes acetylate hisone H3 within nucleosomes, allowing for the recognition and binding of transcriptional activators. Human TAF5L is 589 amino acids (aa) in length. It contains an N-terminal homodimerization interface (aa 90-198) plus six consecutive WD repeats (aa 266-547). There is one potential splice form that shows a Val substitution for aa 325-589. Over aa 1-189, human TAF5L shares 94% aa identity with mouse TAF5L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TAF5L
• Uniprot:
  • O75529(Human)