TAF15 Antibody (4D71)

Images

 
Western Blot: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of cytosol or nuclear enriched fractions of cell line lysates using mouse mAb to TAF15, NBP1-92685, dilution 1:1,000: [1] protein standard, [2] NIH-3T3 ...read more
Immunocytochemistry/ Immunofluorescence: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of HeLa cells stained with mouse mAb to TATA-box binding protein associated factor 15, TAF15, NBP1-92685, dilution 1:500 in red, and ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
4D71
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

TAF15 Antibody (4D71) Summary

Immunogen
Full length recombinant human TAF15 expressed in and purified from E. coli. [UniProt# Q92804]
Localization
Nucleus. Cytoplasm. Note: Shuttles from the nucleus to the cytoplasm.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TAF15
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:500-1:1000
Application Notes
This TAF15 (4D71) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 68 kDa.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-92685.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol (1:1)
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for TAF15 Antibody (4D71)

  • 68kDa
  • hTAFII68
  • Npl3
  • RBP56TAFII68
  • RNA-binding protein 56
  • TAF(II)68
  • TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor
  • TAF2NRBP56/CSMF fusion
  • TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD(RNA-binding protein 56)
  • TATA box-binding protein-associated factor 2N (RNA-binding protein 56)
  • TATA-binding protein-associated factor 2N
  • TBP-associated factor 15,68 kDa TATA-binding protein-associated factor

Background

TAF15 (TAF2N or TAFII68) is a nuclear protein known to associate with a distinct subpopulation of transcription factor IID (TFIID), a multi-subunit complex that nucleates the pre-initiation complex on promoters of on protein-coding genes. TAF15 harbors a transcriptional activation domain, a RNA recognition motif and many Arg-Gly-Gly repeats that participate in RNA binding. TAF15 along with FUS and EWS constitutes the FET (FUS/EWS/TAF15) protein family of heterogeneous nuclear ribonucleoproteins (hnRNPs) class of RNA binding proteins that are multifunctional proteins implicated in numerous aspects of RNA processing/functions. TAF15 is a nuclear protein that shuttle between cytoplasm-nucleus and as RNA/ssDNA-binding protein, it play specific roles during transcription initiation at distinct promoters and enter the preinitiation complex together with the RNA polymerase II. TAF15 as well as other FET member genes are frequently translocated in sarcomas and rare hematopoietic as well as epithelial cancers. Moreover, dominant mutations in genes coding for four hnRNPs (TDP-43, FUS/TLS, TAF15, and EWS) are found in familial and sporadic cases of ALS and a chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TAF15 Antibody (NBP1-92685)(1)

We have publications tested in 1 confirmed species: Human.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP1-92685AF405
Alexa Fluor 488 NBP1-92685AF488
Alexa Fluor 647 NBP1-92685AF647
Alexa Fluor 700 NBP1-92685AF700
DyLight 350 NBP1-92685UV
DyLight 405 NBP1-92685V
DyLight 488 NBP1-92685G
DyLight 550 NBP1-92685R
DyLight 680 NBP1-92685FR
DyLight 755 NBP1-92685IR
FITC NBP1-92685F
Janelia Fluor 549 NBP1-92685JF549
Janelia Fluor 646 NBP1-92685JF646

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Bioinformatics

Gene Symbol TAF15
Entrez
Uniprot