TAF15 Antibody (4D71) [Alexa Fluor™ Plus 488]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP, KO
• Clone: 4D71
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 488

TAF15 Antibody (4D71) [Alexa Fluor™ Plus 488] Summary

• Immunogen: Full length recombinant human TAF15 expressed in and purified from E. coli. [UniProt# Q92804]
• Localization: Nucleus. Cytoplasm. Note: Shuttles from the nucleus to the cytoplasm.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TAF15
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for TAF15 Antibody (4D71) [Alexa Fluor™ Plus 488]

• 68kDa
• hTAFII68
• Npl3
• RBP56TAFII68
• RNA-binding protein 56
• TAF(II)68
• TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor
• TAF2NRBP56/CSMF fusion
• TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD(RNA-binding protein 56)
• TATA box-binding protein-associated factor 2N (RNA-binding protein 56)
• TATA-binding protein-associated factor 2N
• TBP-associated factor 15,68 kDa TATA-binding protein-associated factor

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TAF15