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TAF10 Antibody Pair

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal

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TAF10 Antibody Pair Summary

Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.

  • Antibody pair for IP: rabbit polyclonal anti-TAF10 (300 ul)
  • Antibody pair for WB: mouse polyclonal anti-TAF10 (50 ul)
    		•
    Clonality
    Polyclonal
    Gene
    TAF10

    Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Preservative
    No Preservative

    Notes

    Quality control test: Immunoprecipitation-Western Blot (IP-WB).

    This product is produced by and distributed for Abnova, a company based in Taiwan.

    Alternate Names for TAF10 Antibody Pair

    • 30kDa
    • STAF28
    • TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor
    • TAF2ATAFII-30
    • TAF2Htranscription initiation factor TFIID 30 kD subunit
    • TAFII30TAF(II)30
    • TATA box binding protein (TBP)-associated factor, RNA polymerase II, H, 30kD
    • Transcription initiation factor TFIID 30 kDa subunit
    • transcription initiation factor TFIID subunit 10

    Background

    This antibody pair set comes with matched antibody pair to detect and quantify protein level of human TAF10.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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    Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Staining of Pig Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
    Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - NBP2-12933 (1ug/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Analysis of cell line NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
    Species: Hu, Mu, Rt, Po
    Applications: WB, PEP-ELISA
    TAF8 Antibody
    NBP2-38188
    Western Blot: TAF8 Antibody [NBP2-38188] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative Control. Lane 3: Overexpression lysate
    Immunocytochemistry/Immunofluorescence: TAF8 Antibody [NBP2-38188] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
    Immunohistochemistry: TAF8 Antibody [NBP2-38188] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
    Species: Hu
    Applications: WB, ICC/IF, IHC, IHC-P
    IGFBP-rp1/IGFBP-7
    Antibody [Unconjugate ...
    AF1334
    Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IGFBP‑rp1/IGFBP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1334) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IGFBP‑rp1/IGFBP‑7 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
    IGFBP‑rp1/IGFBP‑7 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human IGFBP‑rp1/IGFBP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1334) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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    KAT2A/GCN5
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    NBP2-66163
    KAT2A/GCN5 Knockout 293T Cell Lysate
    Species: Hu
    PCAF Antibody
    (5E11.)
    H00008850-M04
    Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in A-431 (Cat # L015V1).
    Immunocytochemistry/Immunofluorescence: PCAF Antibody (5E11) [H00008850-M04] - Analysis of monoclonal antibody to PCAF on HeLa cell. Antibody concentration 10 ug/ml
    Western Blot: PCAF Antibody (5E11) [H00008850-M04] - Analysis of PCAF expression in LNCaP (Cat # L004V1).
    (1 Review    )
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    Airway
    Trypsin-like Protease ...
    2695-SE
    Airway Trypsin-like Protease/HAT/TMPRSS11D
    (3 Publications    )
    Species: Hu
    ER alpha/NR3A1
    Antibody (EVG F9)
    NB100-74413
    Western Blot: ER alpha/NR3A1 Antibody (EVG F9) [NB100-74413] - Analysis of various whole cell lysates.
    Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (EVG F9) [NB100-74413] - Analysis of Estrogen Receptor alpha using Anti-Estrogen Receptor alpha Monoclonal Antibody (EVG F9) shows staining in U251 Cells. Estrogen Receptor alpha staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Estrogen Receptor alpha at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
    Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (EVG F9) [NB100-74413] - Cancer biopsies of deparaffinized Human breast carcinoma tissues.
    Species: Hu, Mu, Rb
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    Ataxin 7 Antibody
    NBP1-42657
    Western Blot: Ataxin 7 Antibody [NBP1-42657] - Whole cell lysate (5, 15 and 50 ug) from 293T cells. Antibody used at 0.4 ug/ml.
    (1 Publication    )
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    Applications: IP (-), WB
    CBP/KAT3A Antibody
    NB100-381
    Western Blot: CBP/KAT3A Antibody [NB100-381] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-381 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
    Proximity Ligation Assay: CBP/KAT3A Antibody [NB100-381] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human CBP (NB100-381). Image merged from DAPI (2ms) and Texs Red (200ms) exposures, 40X magnification.
    Proximity Ligation Assay: CBP/KAT3A Antibody [NB100-381] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human CBP (NB100-381). Image merged from DAPI (2ms) and Texs Red (200ms) exposures, 40X magnification.
    (2 Reviews    )
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    PAG1 Antibody
    (PAG-C1)
    NB500-487
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    SETD7/9 Antibody
    NB100-56664
    Western Blot: SETD7/9 Antibody [NB100-56664] - Whole cell extract from MCF7 cells. Image from verified customer review.
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    (1 Publication    )
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    Opsin 1 (Long
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    H00005956-R02
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    HMGB1/HMG-1
    Antibody
    NB100-2322
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    Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody [NB100-2322] - HMGB1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A concentration of 0.05 ug/ml was used. Image objective 40x.
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    NBP1-76729
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    ILK Antibody (4F10)
    H00003611-M01
    Western Blot: ILK Antibody (4F10) [H00003611-M01] - ILK monoclonal antibody (M01), clone 4F10. Analysis of ILK expression in NIH/3T3.
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    Species: Hu, Mu, Rt
    Applications: WB, ELISA, ICC/IF, RNAi
    TAF7 Antibody (2C5)
    H00006879-M01
    Western Blot: TAF7 Antibody (2C5) [H00006879-M01] - TAF7 monoclonal antibody (M01), clone 2C5 Analysis of TAF7 expression in MCF-7.
    Immunocytochemistry/Immunofluorescence: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of monoclonal antibody to TAF7 on HeLa cell. Antibody concentration 10 ug/ml.
    Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1 ug/ml.
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    Applications: WB, ELISA, ICC/IF, IHC-P, RNAi
    Histone H3
    [Trimethyl Lys9] Anti ...
    NBP1-30141
    Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
    Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Histone H3 [Trymethyl Lys9] (6F12-H4) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
    Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Histone 3 was detected in immersion fixed paraffin-embedded sections of human colon cancer using anti-human mouse monoclonal antibody (Catalog # NBP1-30141) at 1:1000 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
    (1 Review    )
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    Species: Hu, Mu, Rt, Po, Ce, Dr, In, Ma, Ye
    Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
    NCOR2 Antibody
    NB120-5802
    Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of U251 cells (right) compared to a negative control without primary antibody (left).
    Immunohistochemistry: NCOR2 Antibody [NB120-5802] - Immunohistochemistry analysis of NCoR2 showing positive staining in the nucleus of paraffin-treated Human lung tissue (right) compared with a negative control in the absence of primary antibody (left).
    Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left).
    Species: Hu, Mu
    Applications: WB, ICC/IF, IHC, IHC-P, IP, ChIP, ICC
    eIF4E [p Ser209]
    Antibody
    NB100-81898
    Western Blot: eIF4E [p Ser209] Antibody [NB100-81898] - Analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using eIF4E (Phospho-Ser209) Antibody.
    Immunocytochemistry/Immunofluorescence: eIF4E [p Ser209] Antibody [NB100-81898] - Staining of methanol-fixed MCF cells using eIF4E(Phospho-Ser209) antibody
    Immunohistochemistry: eIF4E [p Ser209] Antibody [NB100-81898] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using elF4E (phospho-Ser209) antibody
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    Applications: WB, ICC/IF, IHC, IHC-P
    TAF10 Antibody Pair
    H00006881-PW1
    TAF10 Antibody Pair
    Species: Hu
    Applications: WB, IP

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    Bioinformatics

    Gene Symbol TAF10