Recombinant Human TACI/TNFRSF13B/CVID Protein

SDS-Page: Recombinant Human TACI/TNFRSF13B/CVID Protein [NBP2-61308]

• Reactivity: Hu
• Applications: WB, Bioactivity
• Concentration: LYOPH

Recombinant Human TACI/TNFRSF13B/CVID Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 160 amino acids corresponding to TACI/TNFRSF13B/CVID Source: E. coli

  Uniprot ID: O14836

  Amino Acid Sequence: MSGLGRSRRG GRSRVDQEER FPQGLWTGVA MRSCPEEQYW DPLLGTCMSC KTICNHQSQR TCAAFCRSLS CRKEQGKFYD HLLRDCISCA SICGQHPKQC AYFCENKLRS PVNLPPELRR QRSGEVENNS DNSGRYQGLE HRGSEASPAL PGLKLSADQV

• Details of Functionality: Fully biologically active when compared to standard. The biological activity is determined by its ability to block human BAFF induced T2B cell survival using a concentration range of 1.0-3.0 ug/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TNFRSF13B
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of TACI/TNFRSF13B/CVID as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Western Blot
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human TACI/TNFRSF13B/CVID Protein

• CD267 antigen
• CD267
• CVID
• CVID2
• FLJ39942
• MGC133214
• TACI
• TACIMGC39952
• TNFRSF13B
• TNFRSF14B
• Transmembrane activator and CAML interactor
• tumor necrosis factor receptor 13B
• tumor necrosis factor receptor superfamily member 13B
• tumor necrosis factor receptor superfamily, member 13B

Background

TACI is a lymphocyte specific member of the tumor necrosis factor (TNF) receptor superfamily. It interacts with calcium-modulator and cyclophilin ligand (CAML). The protein binds to both APRIL and BAFF and induces activation of the transcription factors NFAT (nuclear-factor of activated T cells), AP1, and NF kappa B and plays a crucial role in humoral immunity by interacting with a TNF ligand. This gene is located within the Smith Magenis syndrome region on chromosome 17.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF13B