TACI/TNFRSF13B/CVID Antibody (165604) [Phycoerythrin]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human TACI/TNFRSF13B PE-conjugated Monoclonal Antibody (Catalog # FAB1741P) and Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TACI/TNFRSF13B PE-conjugated Monoclonal Antibody (Catalog # FAB1741P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 165604
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

TACI/TNFRSF13B/CVID Antibody (165604) [Phycoerythrin] Summary

• Additional Information: Clone 165604 was used by HLDA to establish CD designation
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TACI/TNFRSF13B
  Ser2-Thr166
  Accession # O14836
• Specificity: Detects human TACI/TNFRSF13B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human BCMA or recombinant mouse TACI is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF13B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TACI/TNFRSF13B/CVID Antibody (165604) [Phycoerythrin]

• CD267 antigen
• CD267
• CVID
• CVID2
• FLJ39942
• MGC133214
• TACI
• TACIMGC39952
• TNFRSF13B
• TNFRSF14B
• Transmembrane activator and CAML interactor
• tumor necrosis factor receptor 13B
• tumor necrosis factor receptor superfamily member 13B
• tumor necrosis factor receptor superfamily, member 13B

Background

TACI (Transmembrane Activator and CAML-Interactor) is a 30-32 kDa member of the TNF receptor superfamily. It is classified as is a type III membrane protein with an extracellular N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TACI/TNFRSF13B/CVID Antibody (FAB1741P)(9)

Publications using FAB1741P

• M Jasek, A Bojarska-J, M Sobczy?ski, M Wagner, S Chocholska, J Roli?ski, D Wo?owiec, L Karabon Association of Common Variants of TNFSF13 and TNFRSF13B Genes with CLL Risk and Clinical Picture, as Well as Expression of Their Products-APRIL and TACI Molecules Cancers (Basel), 2020-10-06;12(10):. 2020-10-06 [PMID: 33036273] (Flow Cytometry, Human)
• Johannes Nelke, Juliane Medler, Daniela Weisenberger, Andreas Beilhack, Harald Wajant CD40- and CD95-specific antibody single chain-Baff fusion proteins display BaffR-, TACI- and BCMA-restricted agonism mAbs 2020-08-26 [PMID: 32840410]
• RA Pinna, AC Dos Santos, DS Perce-da-S, LA da Silva, RNR da Silva, MR Alves, F Santos, J de Oliveir, JC Lima-Junio, DM Villa-Verd, PM De Luca, CE Carvalho-P, DM Banic Correlation of APRIL with production of inflammatory cytokines during acute malaria in the Brazilian Amazon Immun Inflamm Dis, 2018-01-03;0(0):. 2018-01-03 [PMID: 29314720] (Flow Cytometry, Human)
• Muema D, Macharia G, Hassan A, Mwaringa S, Fegan G, Berkley J, Nduati E, Urban B Control of Viremia Enables Acquisition of Resting Memory B Cells with Age and Normalization of Activated B Cell Phenotypes in HIV-Infected Children. J Immunol, 2015-06-26;195(3):1082-91. 2015-06-26 [PMID: 26116511] (Flow Cytometry, Human)
• Hoffmann F, Kuhn P, Laurent S, Hauck S, Berer K, Wendlinger S, Krumbholz M, Khademi M, Olsson T, Dreyling M, Pfister H, Alexander T, Hiepe F, Kumpfel T, Crawford H, Wekerle H, Hohlfeld R, Lichtenthaler S, Meinl E The immunoregulator soluble TACI is released by ADAM10 and reflects B cell activation in autoimmunity. J Immunol, 2014-12-10;194(2):542-52. 2014-12-10 [PMID: 25505277] (Flow Cytometry, Human)
• Keiko Yoshimoto, Maiko Tanaka, Masako Kojima, Yumiko Setoyama, Hideto Kameda, Katsuya Suzuki et al. Regulatory mechanisms for the production of BAFF and IL-6 are impaired in monocytes of patients of primary Sjögren's syndrome Arthritis Research & Therapy 2011-10-21 [PMID: 22018243]
• Bojarska-Junak A, Hus I, Chocholska S, Wasik-Szczepanek E, Sieklucka M, Dmoszynska A, Rolinski J BAFF and APRIL expression in B-cell chronic lymphocytic leukemia: correlation with biological and clinical features. Leuk. Res., 2009-04-23;33(10):1319-27. 2009-04-23 [PMID: 19395025] (Flow Cytometry, Human)
• Sarantopoulos S, Stevenson KE, Kim HT, Cutler CS, Bhuiya NS, Schowalter M, Ho VT, Alyea EP, Koreth J, Blazar BR, Soiffer RJ, Antin JH, Ritz J Altered B-cell homeostasis and excess BAFF in human chronic graft-versus-host disease. Blood, 2009-01-23;113(16):3865-74. 2009-01-23 [PMID: 19168788] (Flow Cytometry, Human)
• Tai YT, Li XF, Breitkreutz I, Song W, Neri P, Catley L, Podar K, Hideshima T, Chauhan D, Raje N, Schlossman R, Richardson P, Munshi NC, Anderson KC Role of B-cell-activating factor in adhesion and growth of human multiple myeloma cells in the bone marrow microenvironment. Cancer Res., 2006-07-01;66(13):6675-82. 2006-07-01 [PMID: 16818641] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TNFRSF13B
• Uniprot:
  • O14836()