TAB2 Overexpression Lysate (Native)

Images

 
Western Blot: TAB2 Overexpression Lysate (Adult Normal) [NBL1-12857] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TAB2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TAB2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207721 and based on accession number NM_015093. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens mitogen-activated protein kinase kinase kinase 7 interacting protein 2 (MAP3K7IP2), mRNA.
Gene
TAB2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
76.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for TAB2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TAB2 Overexpression Lysate (Native)

  • FLJ21885
  • KIAA0733CHTD2
  • MAP3K7IP2mitogen-activated protein kinase kinase kinase 7 interacting protein 2
  • Mitogen-activated protein kinase kinase kinase 7-interacting protein 2
  • TAB-2
  • TAK1-binding protein 2
  • TGF-beta activated kinase 1/MAP3K7 binding protein 2
  • TGF-beta-activated kinase 1 and MAP3K7-binding protein 2
  • TGF-beta-activated kinase 1-binding protein 2

Background

The protein encoded by this gene is an activator of MAP3K7/TAK1, which is required for for the IL-1 induced activation of nuclear factor kappaB and MAPK8/JNK. This protein forms a kinase complex with TRAF6, MAP3K7 and TAB1, thus serves as an adaptor linking MAP3K7 and TRAF6. This protein, TAB1, and MAP3K7 also participate in the signal transduction induced by TNFSF11/RANKl through the activation of the receptor activator of NF-kappB (TNFRSF11A/RANK), which may regulate the development and function of osteoclasts. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for TAB2 Lysate (NBL1-12857) (0)

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Video Protocols

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Bioinformatics

Gene Symbol TAB2