Syntenin 1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody [NBP1-31136] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Syntenin 1antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.

Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-31136] - Mouse liver. Syntenin 1 antibody [N1], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunoprecipitation: Syntenin 1 Antibody [NBP1-31136] - Immunoprecipitation of Syntenin 1 protein from U87-MG whole cell extracts using 5 ug of Syntenin 1 antibody [N1], N-term (NBP1-31136). Western blot analysis was performed using Syntenin 1 antibody [N1], N-term (NBP1-31136).EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Pm, RM, Xp
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Syntenin 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Syntenin 1 Antibody - BSA Free (NBP1-31136) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Syntenin 1. The exact sequence is proprietary.
• Localization: Cell junction, focal adhesion, adherens junction, Cell membrane, Peripheral membrane protein, Endoplasmic reticulum membrane, Peripheral membrane protein, Nucleus, Melanosome, Cytoplasm, cytosol, cytoskeleton
• Predicted Species: Rat (100%), Porcine (100%), Chimpanzee (100%), Bovine (100%), Rhesus Macaque (100%), Chicken (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SDCBP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Assay dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Syntenin 1 Antibody - BSA Free

• MDA9
• MDA-9
• melanoma differentiation associated protein-9
• Melanoma differentiation-associated protein 9
• Pro-TGF-alpha cytoplasmic domain-interacting protein 18
• Scaffold protein Pbp1
• ST1
• SYCLTACIP18
• syndecan binding protein (syntenin)
• Syndecan-binding protein 1
• syntenin-1

Background

The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SDCBP