Syntaxin 7 Antibody (CL0257)

Analysis in human tonsil tissue, human cell line RT-4 and human cell line HEK 293.

Staining of human malignant melanoma shows moderate to strong cytoplasmic positivity in tumor cells.

Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

Staining of U-251 cells showing specific staining in vesicles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Staining of human lymph node shows moderate to strong cytoplasmic positivity in lymphoid cells.

Analysis in human lymph node and testis tissues. Corresponding Syntaxin 7 RNA-seq data are presented for the same tissues.

Staining of human kidney shows strong apical membrane positivity in cells in proximal tubules.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0257
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Syntaxin 7 Antibody (CL0257) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was developed using a recombinant protein derived from O15400, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: STX7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, 40% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Syntaxin 7 Antibody (CL0257)

• STX7
• Syntaxin 7
• syntaxin-7

Background

Syntaxin 7 is involved in the ordered fusion of endosomes and lysosomes with the phagosome, where phagocytic cells kill and degrade internalized foreign particles. Syntaxin 7 is found in late endosomes and lysosomes; whereas Syntaxin 12 is localized to the recycling endosome compartment, both are recruited to the phagosome. However, STX12 is acquired earlier before rapidly recycling off the phagosome, whereas STX7 is recruited later and continues to accumulate throughout the phagosome maturation process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STX7