Syntaxin 6 Antibody

Images

 
Western Blot: Syntaxin 6 Antibody [NB100-2920] - HEK293 overexpressing Human STX6 and probed with (mock transfection in first lane).
Western Blot: Syntaxin 6 Antibody [NB100-2920] - Western blot analysis of STX6using NB100-2920 at 0.3ug/ml in Human Lymph Node lysate (35ug protein in RIPA buffer).
Western Blot: Syntaxin 6 Antibody [NB100-2920] - (0.3ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Syntaxin 6 Antibody Summary

Immunogen
Peptide with sequence C-QALAERKNRQA corresponding to internal region according to NP_005810.1.
Marker
TGN Marker
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
STX6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 30 kDa band observed in human lymph node lysates (calculated MW of 29.2 kDa band according to NP_005810.1). In transfected HEK293 transiently expressing human STX6 a band of approx. 30 kDa is observed. This band is not observed in the non-transfected HEK293.
Positive Control
Syntaxin 6 Lysate (NBL1-16584)
Publications
Read Publication using NB100-2920.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Syntaxin 6 Antibody

  • STX6
  • Syntaxin 6
  • syntaxin-6

Background

Syntaxin 6 is a 35 kDa type II integral membrane protein with two coiled coil domains and a carboxyl terminal membrane anchor. Immunoelectron microscopy studies show that syntaxin 6 is primarily localized to the trans Golgi network (TGN), and is partially co localized with AP 1 on clathrin coated membranes. During secretory granule maturation, syntaxin 6 and MPR ligand complexes are postulated to be transferred from immature granules to endosomes by clathrin coated vesicles, as evidenced by immunoelectron microscopy. These results suggest that syntaxin 6 mediates a TGN trafficking targeting endosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Syntaxin 6 Antibody (NB100-2920)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STX6
Entrez
Uniprot