Syntaxin 6 Antibody

Western Blot: Syntaxin 6 Antibody [NB100-2920] - HEK293 overexpressing Human STX6 and probed with (mock transfection in first lane).

Western Blot: Syntaxin 6 Antibody [NB100-2920] - Western blot analysis of STX6using NB100-2920 at 0.3ug/ml in Human Lymph Node lysate (35ug protein in RIPA buffer).

Western Blot: Syntaxin 6 Antibody [NB100-2920] - (0.3ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Syntaxin 6 Antibody Summary

• Immunogen: Peptide with sequence C-QALAERKNRQA corresponding to internal region according to NP_005810.1.
• Marker: TGN Marker
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: STX6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 30 kDa band observed in human lymph node lysates (calculated MW of 29.2 kDa band according to NP_005810.1). In transfected HEK293 transiently expressing human STX6 a band of approx. 30 kDa is observed. This band is not observed in the non-transfected HEK293.
• Control:

  Syntaxin 6 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Syntaxin 6 Antibody

• STX6
• Syntaxin 6
• syntaxin-6

Background

Syntaxin 6 is a 35 kDa type II integral membrane protein with two coiled coil domains and a carboxyl terminal membrane anchor. Immunoelectron microscopy studies show that syntaxin 6 is primarily localized to the trans Golgi network (TGN), and is partially co localized with AP 1 on clathrin coated membranes. During secretory granule maturation, syntaxin 6 and MPR ligand complexes are postulated to be transferred from immature granules to endosomes by clathrin coated vesicles, as evidenced by immunoelectron microscopy. These results suggest that syntaxin 6 mediates a TGN trafficking targeting endosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Syntaxin 6 Antibody (NB100-2920)(1)

Publication using NB100-2920

• Murray RZ, Wylie FG, Khromykh T et al. Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alpha. J Biol Chem 2005-03-18 [PMID: 15640147]

Bioinformatics

• Gene Symbol: STX6
• Entrez:
  • 10228(Human)
  • 58244(Mouse)
  • 60562(Rat)
• Uniprot:
  • NP_005810.1(Human)