Syntabulin/GOLSYN Antibody

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Syntabulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5544) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Syntabulin at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Syntabulin/GOLSYN Antibody Summary

• Immunogen: E. coli-derived recombinant human Syntabulin
  Gln489-Arg656
  Accession # Q9NX95
• Specificity: Detects human Syntabulin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SYBU
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Syntabulin/GOLSYN Antibody

• Golgi-Localized Protein
• Golgi-Localized Syntaphilin-Related Protein
• GOLSYN A Protein
• GOLSYN B Protein
• GOLSYN C Protein
• GOLSYN
• Implicated In Syntaxin Trafficking In Neurons
• KIAA1472
• Microtubule-Associated Protein
• OCSYN
• SNPHL
• SYBU
• Syntabulin (Syntaxin-Interacting)
• Syntabulin
• syntaphilin-like
• Syntaxin-1-Binding Protein

Background

Syntabulin (also Golgi-localized synaphilin-related protein) is a 70-75 kDa molecule that belongs to the syntaxin-binding and kinesin-1 family of motor adaptor proteins. It is highly spliced, with various forms associated with different tissues. In neurons, it anchors syntaxin-1 to KIF5B, creating a complex by which synaptic components can be transported to synaptic terminals. Human Syntabulin is a type II transmembrane (TM) protein 663 amino acids (aa) in length. It contains one coiled-coil region (aa 271‑353) and a TM domain between aa 606‑626. Alternate splice forms show alternate start sites at Met120 and Met207, a five aa substitution for aa 1‑8, a 16 aa insertion after Lys8, and an eight aa substitution for aa 1‑76. Over aa 486‑653, human Syntabulin shares 83% aa identity with mouse Syntabulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYBU
• Uniprot:
  • Q9NX95()