Rat Syndecan-4 ELISA Kit (Colorimetric)

ELISA: Rat Syndecan-4 ELISA Kit (Colorimetric) [NBP2-76613] - Samples were spiked with high concentrations of Rat Syndecan-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Syndecan-4 ELISA Kit (Colorimetric) [NBP2-76613] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.313 - 20ng/mL
• Sensitivity: 0.188ng/mL

Rat Syndecan-4 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat SDC4 in samples. No significant cross-reactivity or interference between Rat SDC4 and analogues was observed.
• Standard Curve Range: 0.313 - 20ng/mL
• Sensitivity: 0.188ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.38
• Intra-Assay: CV% < 4.12
• Spike Recovery: 84-104%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SDC4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Syndecan-4 ELISA Kit (Colorimetric)

• Amphiglycan
• MGC22217
• Ryudocan core protein
• Ryudocan
• SDC4
• SYND4ryudocan amphiglycan
• syndecan 4 (amphiglycan, ryudocan)
• syndecan 4
• syndecan proteoglycan 4
• Syndecan4
• Syndecan-4

Background

Syndecans are type I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan-1 is an matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan-1 and Syndecan-3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan-2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan-4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Syndecan-4 ELISA Kit (NBP2-76613). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SDC4