Syndecan-4 Antibody

Western Blot: Syndecan-4 Antibody [NB110-41551] - Analysis of Syndecan-4 with Jurkat cell lysate.

Immunohistochemistry-Paraffin: Syndecan-4 Antibody [NB110-41551] - FFPE tissue after heat-induced antigen retrieval. 5 ug/mL of antibody used for staining. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Product Discontinued

Syndecan-4 Antibody Summary

• Description: This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: 3644-100)
• Immunogen: Synthetic peptide surrounding amino acid 184 of human Syndecan-4.
• Marker: Plasma Membrane Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SDC4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Frozen 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Immunoprecipitation 5 - 10 ug/mL
  • Western Blot 0.5 - 4 ug/mL
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Jurkat Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20 °C.
• Buffer: PBS (pH 7.2), 0.5% BSA, 30% Glycerol
• Preservative: 0.01% Thimerosal
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for Syndecan-4 Antibody

• Amphiglycan
• MGC22217
• Ryudocan core protein
• Ryudocan
• SDC4
• SYND4ryudocan amphiglycan
• syndecan 4 (amphiglycan, ryudocan)
• syndecan 4
• syndecan proteoglycan 4
• Syndecan4
• Syndecan-4

Background

Syndecans are type I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan-1 is an matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan-1 and Syndecan-3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan-2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan-4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Syndecan-4 Antibody (NB110-41551)(2)

Publications using NB110-41551

• Stavely R, Hotta R, Guyer RA et al. A distinct transcriptome characterizes neural crest-derived cells at the migratory wavefront during enteric nervous system development Development (Cambridge, England) 2023-02-13 [PMID: 36779913]
• Cui C, Pan Y, Zhang C et al. Eltrombopag binds SDC4 directly and enhances MAPK signaling and macropinocytosis in cancer cells American journal of cancer research 2022-06-15 [PMID: 35812066] (WB, Human)

Review for Syndecan-4 Antibody (NB110-41551) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 03/17/2017

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult mouse brain tissue
• Species: Mouse
• Lot: 2M21L36449

Comments

• Comments: Mouse brain cells were sorted by flow cytometry then leaved 4h at 37°C on polyDlysine treated slides.
  4% Paraformaldeyde fixed cells and permeabilized with 0.1% triton -X100. Antibody used at 5µg/ml.

Bioinformatics

• Gene Symbol: SDC4
• Entrez:
  • 6385(Human)
  • 20971(Mouse)
  • 24771(Rat)
• Uniprot:
  • P31431()