Syndecan-4 Antibody

Images

 
Western Blot: Syndecan-4 Antibody [NB110-41551] - Analysis of Syndecan-4 with Jurkat cell lysate.
Immunohistochemistry-Paraffin: Syndecan-4 Antibody [NB110-41551] - FFPE tissue after heat-induced antigen retrieval. 5 ug/mL of antibody used for staining. After incubation with the primary antibody, slides were ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Syndecan-4 Antibody Summary

Immunogen
Synthetic peptide surrounding amino acid 184 of human Syndecan-4.
Marker
Plasma Membrane Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SDC4
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 4 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Frozen 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Immunoprecipitation 5 - 10 ug/mL
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Jurkat Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB110-41551 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20 °C.
Buffer
PBS (pH 7.2), 0.5% BSA, 30% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for Syndecan-4 Antibody

  • Amphiglycan
  • MGC22217
  • Ryudocan core protein
  • Ryudocan
  • SDC4
  • SYND4ryudocan amphiglycan
  • syndecan 4 (amphiglycan, ryudocan)
  • syndecan 4
  • syndecan proteoglycan 4
  • Syndecan4
  • Syndecan-4

Background

Syndecans are type I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan-1 is an matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan-1 and Syndecan-3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan-2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan-4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Syndecan-4 Antibody (NB110-41551) (0)

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Review for Syndecan-4 Antibody (NB110-41551) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB110-41551:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Syndecan-4 NB110-41551
Enlarge
5
reviewed by:
Anonymous
IHC-Fr Mouse 03/17/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult mouse brain tissue
SpeciesMouse
Lot2M21L36449

Comments

CommentsMouse brain cells were sorted by flow cytometry then leaved 4h at 37°C on polyDlysine treated slides.
4% Paraformaldeyde fixed cells and permeabilized with 0.1% triton -X100. Antibody used at 5µg/ml.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Syndecan-4 Antibody (NB110-41551) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Syndecan-4 Antibody (NB110-41551)

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Recent Reviews

5
5
1
4
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3
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2
0
1
0

Anonymous
03/17/2017
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol SDC4
Entrez
Uniprot