Syndecan-4 Antibody (336304) [Unconjugated]

HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Syndecan-4 Monoclonal Antibody (Catalog # MAB29181, filled histogram) or Rat IgG2A isotype control antibody (Catalog # MAB006, open histogram) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 336304
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Syndecan-4 Antibody (336304) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Syndecan-4
  Ser20-Glu145
  Accession # P31431
• Specificity: Detects human Syndecan-4 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Syndecan-4 Antibody (336304) [Unconjugated]

• Amphiglycan
• MGC22217
• Ryudocan core protein
• Ryudocan
• SDC4
• SYND4ryudocan amphiglycan
• syndecan 4 (amphiglycan, ryudocan)
• syndecan 4
• syndecan proteoglycan 4
• Syndecan4
• Syndecan-4

Background

Syndecan-4, previously known as Amphiglycan or Ryudocan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying Heparan Sulfate (HS) and Chondroitin Sulfate (CS) glycosaminoglycans. The four vertebrate syndecans have two conserved cytoplasmic domains and divergent extracellular portions, except for HS attachment sites. Syndecan-4 is the most similar to Syndecan-2, but is more universally expressed and is found in virtually every cell type. Expression can be upregulated by TGF-beta 2 and in response to mechanical stress in smooth muscle, wound healing, arterial injury or acute myocardial infarction, probably in response to at least one inflammatory mediator (1, 2). Human Syndecan-4 is synthesized as a 198 amino acid (aa) core protein with an 18 aa signal sequence, a 127 aa extracellular domain containing three consensus Ser-Gly sequences for the attachment of HS side chains, a 25 aa transmembrane region and a 28 aa cytoplasmic tail (3). Human Syndecan-4 ECD shares approximately 79%, 78% and 81% aa sequence identity with mouse, rat and porcine Syndecan-4 ECD, respectively. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 20 kDa core protein. Non-covalent homodimerization of Syndecan-4 is dependent on the transmembrane domain (4). The HS chains can bind fibronectin, SDF-1, antithrombin, FGF-2, midkine and tissue factor pathway inhibitor and can present FGF‑2 to its receptors (1, 2, 5). Proteolytic cleavage by plasmin, thrombin or a metalloproteinase may create a functional ectodomain (6‑8). Genetic disruption of the Syndecan-4 gene causes a mild phenotype, presumably due to compensation by other syndecans, but mice have an increase in placental thrombi as well as defects in wound healing and response to endotoxin shock (9, 10).

1. Tkachenko, E. et al. (2005) Circ. Res. 96:488. 
2. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
3. David, G. et al. (1992) J. Cell Biol. 118:961. 
4. Choi, S. et al. (2005) J. Biol. Chem. 280:42573. 
5. Charnaux, N. et al. (2005) FEBS J. 272:1937.  
6. Schmidt, A. et al., J. Biol. Chem. 280:34441.
7. Rauch, B. H. et al. (2005) J. Biol. Chem. 280:17507.
8. Fitzgerald, M. L. et al. (2000) J. Cell Biol. 148:811.
9. Ishiguro, K. et al. (2003) Glycoconj. J. 19:315.
10. Echtermeyer, F. et al. (2001) J. Clin. Invest. 107:R9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P31431()