![Western Blot: Synaptojanin 1 Antibody (5H1) [NBP1-47559] - Rat Hippocampal Lysate showing specific immunolabeling of the ~145k Synaptojanin protein.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-5H1-Western-Blot-NBP1-47559-img0001.jpg "Western Blot: Synaptojanin 1 Antibody (5H1) [NBP1-47559] - Rat Hippocampal Lysate showing specific immunolabeling of the ~145k Synaptojanin protein.")
| Reactivity | RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | 5H1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide Free |
| Description | Novus Biologicals Mouse Synaptojanin 1 Antibody (5H1) - Azide Free (NBP1-47559) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant protein from the C-terminal region of rat synaptojanin 1. Accession # Q62910 |
| Specificity | Specific for endogenous levels of the ~145 kDa synaptojanin I protein in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SYNJ1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 145 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Preservative | No Preservative |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")