Surf6 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Surf6 Antibody [NBP1-83550] - Staining of human cell line U-251 MG shows localization to nucleus & nucleoli. Antibody staining is shown in green.

Staining of human liver shows strong positivity in nucleoli in hepatocytes.

Staining of human cervix, uterine shows strong positivity in nucleoli in squamous epithelial cells.

Staining of human skeletal muscle shows strong positivity in nucleoli in myocytes.

Staining of human colon shows strong positivity in nucleoli in glandular cells and lymphoid cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Surf6 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: AKDAYLQSLAKKICSHSAPEQQARTRAGKTQGSETAGPPKKKRKKTQKKFRKREEKAAEHKAKSLGEKSPAASGARRPEAAKEEAAWASSSAGNPADGLATEPESVFALDVLRQRLHEKIQE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SURF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Surf6 Protein (NBP1-83550PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Surf6 Antibody - BSA Free

• FLJ30322
• RRP14
• SURF-6
• surfeit 6
• surfeit locus protein 6

Background

Surf6 is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. The gene demonstrates features of a housekeeping gene, being ubiquitously expressed, and the encoded protein has been localized to the nucleolus. The protein includes motifs found in both the mouse and fish orthologs, which suggests a putative function as a nucleolar-matrix protein with nucleic acid-binding properties, based on characteristics determined in mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SURF6